FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144599
Sequences flagged as poor quality0
Sequence length150
%GC37

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGATCTCACAGAAACTTCCTTGATGTTCGCACCCAAGCTCTATGTGT1574510.888733670357333No Hit
CATAGAGGGGCCTGGTTATTACTTGGAGGCTGGAGAAAAAGCTCTATGTG83575.779431393024848No Hit
CATAGAGCTCATTCAACTAATAACTGGTAGACCATGGAGAGAATAATCCG78175.405984827004336No Hit
CATAGATTCTTTCCTGGCTGTGGGTGGGTTGAGGGAGTCTTCTCTATGTG68194.715800247581242No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA46823.237920040940809No Hit
CATAGAAGTATTCACACTGGGGCCCAGAACCCATTGTAGATTTCTTTCAC43122.982039986445273No Hit
CATAGAAGGAACAGCAGAGAGGGCAGGCCCTTCAGCATCATTTCTATGTG41842.8935193189441146No Hit
CATAGAATCTGGTTCAAGCACACAGTTGTGGAAAGTGCAATGAGTCTATG38222.643171806167401No Hit
CATAGAGCTAATAAAGACATACCTGAGCCATGCTGAAGTCCCATGATCTA37642.603060878705939No Hit
CATAGATGGTATTTGAGTGGGGAAACAGGCCCTTCAGCATCATTTCTATG32682.26004329213895No Hit
CATAGATGGGACAGCGACAATGACAGTTGTGGAAAGTGCAATGAGTCTAT30982.1424767806139737No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACTGTTAATATAGAAGAGACACA28561.975117393619596No Hit
CATAGACCTCCCATGGTTCCTGATGAGCATGCCTTTGATAAAACATCTAT25731.779403730316254No Hit
CATAGATGAAGGCAGAAAAGGAAGAACAACAACAAAACTCGTGACTTCTA24791.7143963651200906No Hit
CATAGATCTCACAGAAACTTCCTTGATGAGCATGCCTTTGATAAAACATC24671.7060975525418571No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGATGGGAGTGTCTGCTGGTC24641.7040228493972989No Hit
CATAGAACACGAAGATTTCAGATTAGCCTCTCCAGTTTCTACGTGATCTA23051.5940635827357035No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACAGTTGTGGAAAGTGCAATGAG19951.3796775911313357No Hit
CATAGATCTAGCCCATCACTTCAGCAGGCCCTTCAGCATCATTTCTATGT18061.2489712930241565No Hit
CATAGAGGGGTAATACTTCCTACTTCTGCATTAGGAAGTCTTCTCAGCTT16081.112040885483302No Hit
CATAGAAGTATTCACACTGGGGCCCATTGTAGATTTCTTTCACCTCCTCT15131.0463419525722861No Hit
CATAGATTCAGAGAAGGACAGCGTGGGCTGGTGTTTTCCTTGATCTATGT13040.901804300168051No Hit
CATAGAACATTGATTCCTGGCATGTCAGACCCAGGCTACACATCTATGTG11390.7876956272173391No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGTAGAACCATGT11380.7870040595024861No Hit
CATAGACCAGTACGCCTTACCCCTTGGAGGCTGGAGAAAAAGCTCTATGT11050.7641823249123438No Hit
CATAGATTCAGAGAAGGACAGCGTGGGTTGAGGGAGTCTTCTCTATGTGT10810.7475846997558766No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGAGCATGCCTTTGATAAAAC10770.7448184288964654No Hit
CATAGACCCTCTCTTACCCTTCCACCAACATGCTTTTAGTCACGTCTATG8900.6154952662189919No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTACCAACATGCTTTTAGT8340.5767674741872351No Hit
CATAGATGGCCCAGAATCCTTTGTAGATTTCTTTCACTCTCCCACCATTC8180.5657023907495903No Hit
CATAGATTCAGTTTACTTAGAGGCAGACAGTTGTGGAAAGTGCAATGAGT7780.5380396821554783No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTAGCCCGTTCTCTCTGTT7360.5089938381316607No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTACGAATGAGGAAACAGG6940.4799479941078431No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTACCCTGGGAGAAGAAAT6860.47441545238902066No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTGGGCTGGTGTTTTCCTT6730.4654250720959343No Hit
CATAGACCTCCCATGGTTCCTGATGATGGGAGTGTCTGCTGGTCTATGTG6530.4515937177988783No Hit
CATAGAGCAACAATGAAGGTGCATAAAAACTGTCGGAGGTCTATGTGTAG5900.40802495176315184No Hit
CATAGATGGCAAATGGTGGGATCTCTATGTGTAGAACCATGTCGTCAGTG5340.3692971597313951No Hit
CATAGAACATTGATTCCTGGCATGTCCCATGATTTACCATCACAAATTTT5280.3651477534422783No Hit
CATAGACCCTCTCTTACCCTTCCACCCTGGGAGAAGAAATGTTGGTCTAT5270.3644561857274255No Hit
CATAGACCCCATGACTCAGCTCCTCAATCCTGTGTTCATGGCTTCTATGT5040.3485501282858111No Hit
CATAGATTCCTCCAAAGACTGCAGGGGTCAGTCTTCAGCCCACCCATGCC4840.3347187739887551No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACAGTGCATAAAAACTGTCGGAG4670.3229621228362575No Hit
CATAGAGCAACCCCAGCAGTTTGAGAAAAGATGCAGTACAGCTCTATGTG4640.3208874196916991No Hit
CATAGATCTCCTCTCTGTAATGCACCAGTGTATGGGTCATTGTGAGGATT4500.3112054716837599No Hit
CATAGATTCTTTCCTGGCTGTGGGTGTATGAGCATGCCTTTGATAAAACA4240.29322471109758713No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCTATGTGTAGAACCATG4020.2780102213708255No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTATGTGCTCGTATCTTGG3820.26417886707376953No Hit
CATAGATGGCAAATGGTGGGATCTCTATGTGCTCGTATCTTGGCCATCTA3820.26417886707376953No Hit
CATAGATTCTTTCCTGGCTGTGGGTGCATAAAAACTGTCGGAGGTCTATG3490.24135713248362714No Hit
CATAGAGCTAATAAAGACATACCTGAGCCAGTGTATGAGCATGCCTTTGA3120.21576912703407353No Hit
CATAGATGGCCCAGAATCCTTTGTAGATGACTGTTAATATAGAAGAGACA3080.21300285617466236No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGCAGGCCCTTCA2950.20401247588157592No Hit
CATAGAAGTCTCGCTCTGTCATGCAACAACAACAAAACTCGTGACTTCTA2930.20262934045187034No Hit
CATAGAAGGAACAGCAGAGAGGGCAGTGTACAGTTGTGGAAAGTGCAATG2870.19847993416275356No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACGAATGAGGAAACAGGCACTC2870.19847993416275356No Hit
CATAGATCTAGCCCATCACTTCAGCAGCAGGCCCTTCAGCATCATTTCTA2740.18948955386966715No Hit
CATAGATGGCAAATGGTGGGATCTCTACACTGTCATTGTGGGTTTTAACT2690.18603171529540313No Hit
CATAGATCTCACAGAAACTTCCTTGATGATGGGAGTGTCTGCTGGTCTAT2560.17704133500231675No Hit
CATAGACCAGTACGCCTTACCCCTTGTCAGACCCAGGCTACACATCTATG2540.17565819957261114No Hit
CATAGACCAGTACGCCTTACCCCTTGTTATAGAAAACAAGGAGAACATCT2490.17220036099834715No Hit
CATAGAGCTCATTCAACTAATAACTGGTAGACCCTGGGAGAAGAAATGTT2290.15836900670129114No Hit
CATAGATGGCCCAGAATCCTTTGTAGACTGTTAATATAGAAGAGACACAC2280.15767743898643835No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACCCTTCAGCATCATTTCTATG2130.14730392326364636No Hit
CATAGAACTTTTTCAGAGACAGTTGGGACTGTTAATATAGAAGAGACACA1960.13554727211114878No Hit
CATAGAGCAACCCCAGCAGTCTGAGAAAAGATGCAGTACAGCTCTATGTG1950.13485570439629596No Hit
CATAGATGGCAAATGGTGGGATCTCTATGGCAAATGGTGGGTCCTAAATG1940.13416413668144317No Hit
CATAGATGGGACAGCGACAATGACAGTGCATAAAAACTGTCGGAGGTCTA1930.13347256896659038No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCTATGTGGTAAAGGCTG1920.13278100125173756No Hit
CATAGATCTAGCCCATCACTTCAGCATCGCAGGCCCTTCAGCATCATTTC1840.12724845953291516No Hit
CATAGACCTCCCATGGTTCCTGATGATAGACCTCCCATGATTTACCATCA1810.12517375638835676No Hit
CATAGATGGCCCAGAATCCTTTGTAGAACACAGTTGTGGAAAGTGCAATG1780.12309905324379836No Hit
CATAGAGCTAATAAAGACATACCTGAGCCATGTCGTCAGTGTATGCCTGG1720.11894964695468156No Hit
CATAGAGGCTGCAGTGAACAATGATCGTCAGTGTACCCTGGGAGAAGAAA1660.11480024066556477No Hit
CATAGACCCTCTCTTACCCTTCCACCATTTCACTCTCCCACCATTCAGAC1600.11065083437644797No Hit
CATAGATGGGACAGCGACAATGACAGTGTGTCTGAGAAAAGATGCAGTAC1600.11065083437644797No Hit
CATAGAGCTAATAAAGACATACCTGAGCCATGTCGTCAGTGTACCCTGGG1590.10995926666159517No Hit
CATAGATCTAGCCCATCACTTCAGCACTCTCCCACCATTCAGACTCTATG1550.10719299580218397No Hit
CATAGAACTTTTTCAGAGACAGTTGGGACAGTTGTGGAAAGTGCAATGAG1520.10511829265762557No Hit
CATAGATTCCTCCAAAGACTGCAGGCCCTTCAGCATCATTTCTATGTGTA1520.10511829265762557No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)