FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185222
Sequences flagged as poor quality0
Sequence length150
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA50432.7226787314681844No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA49912.694604312662643No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA48092.5963438468432476No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA48022.592564598157886No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA38652.0866851669888025No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG14080.7601688784269687No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG13780.7439720983468486No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12990.7013205774691991No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12820.6921424020904644No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12550.6775653000183564No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG12480.673786051332995No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA11950.6451717398581162No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11250.6073792530045027No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG10970.5922622582630573No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG10960.5917223655937199No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10620.5733660148362505No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG10320.5571692347561304No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT8890.47996458304089146No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT8720.47078640766215674No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA8670.4680869443154701No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8440.4556694129207114No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT7960.4297545647925193No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7910.4270551014458326No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT7800.4211162820831219No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT7260.3919620779389057No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT7160.38656315124553237No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6790.366587122480051No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6310.3406722743518588No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5790.3125978555463174No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT5760.3109781775383054No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5750.310438284868968No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5430.2931617194501733No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5380.2904622561034866No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG5370.2899223634341493No Hit
NATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA5150.27804472470872793No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4970.26832665666065586No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4940.2667069786526438No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4940.2667069786526438No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA4420.23863255984710238No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4380.23647298916975304No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA4160.22459535044433168No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4110.221895887097645No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4030.2175767457429463No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3970.2143373897269223No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3940.21271771171891027No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3890.21001824837222363No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3860.20839857036421158No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3830.20677889235619962No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3770.2035395363401756No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3720.2008400729934889No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3640.19652093163879022No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3420.1846432929133688No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3420.1846432929133688No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3370.18194382956668215No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3330.1797842588893328No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3230.17438533219595945No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3230.17438533219595945No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3110.16790662016391142No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3070.16574704948656208No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2920.15764865944650203No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2760.14901037673710468No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2720.1468508060597553No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2610.14091198669704463No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACAACACAGGATCCAGGTGTC2590.13983220135836996No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2590.13983220135836996No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2420.13065402597963524No Hit
NNNNGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC2400.12957424064096057No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT2360.12741466996361123No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC2310.12471520661692455No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT2300.12417531394758723No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC2230.12039606526222588No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACAACACAGGATCCAGGTGTC2230.12039606526222588No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACAACACAGGATCCAGGTGTC2180.11769660191553918No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTCAGTTTCCCCTCCTTCTATGT2170.11715670924620186No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC2130.1149971385688525No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAGAGAAAACTACCCCAGGCTC2100.11337746056084051No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACAACACAGGATCCAGGTGTC2060.11121788988349116No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2050.11067799721415383No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2050.11067799721415383No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTCAGTTTCCCCTCCTTCTATGT2020.10905831920614183No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2020.10905831920614183No Hit
NNTAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2010.10851842653680449No Hit
NNTAGNTGACCTGTTAATCTGCCCCCGTACCATCAATTTAAGGTAGGCAC2010.10851842653680449No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1990.10743864119812982No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1990.10743864119812982No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1980.10689874852879248No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1960.1058189631901178No Hit
NNNAGNTGACCTGTTAATCTGCCCCCGTACCATCAATTTAAGGTAGGCAC1940.10473917785144313No Hit
NNTAGNTGATGTGAAAAGGACCCATGGCCTGTGTTCTTTGCATGATCTAT1930.10419928518210579No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1920.10365939251276847No Hit
NNTNGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1860.10042003649674446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)