FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808345

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808345
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87686
Sequences flagged as poor quality0
Sequence length150
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17371.980931961772689No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA17011.9398763770727367No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16371.8668886709394885No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16181.8452204456811807No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA12661.4437880619483157No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG12081.3776429532650594No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11291.2875487535068313No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10241.1678032981319708No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9481.0811303970987387No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG9471.0799899641904067No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG9231.0526195743904387No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9001.0263896174988025No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8410.9591040759072144No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7060.8051456332823941No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6830.7789156763907579No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6550.7469835549574618No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6480.7390005245991378No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5950.6785575804575417No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5860.6682936842825536No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5440.6203955021326095No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5400.6158337704992816No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4870.5553908263576853No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4830.5508290947243574No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4810.5485482289076933No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4770.5439864972743654No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4550.5188969732910613No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4550.5188969732910613No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4430.5052117783910772No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4410.5029309125744132No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT4350.49608831512442125No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4310.49152658349109324No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4100.4675774924161212No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4010.4573135962411331No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3990.45503273042446907No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3850.4390666697078211No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3840.43792623679948905No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3840.43792623679948905No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3840.43792623679948905No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3770.4299432064411651No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3620.412836712816185No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3540.40371324954952903No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3280.37406199393289696No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3200.36493853066624093No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3180.3626576648495769No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3070.35011290285792485No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3020.34441073831626484No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2990.3409894395912689No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2930.3341468421412768No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2920.33300640923294483No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2900.33072554341628085No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2890.3295851105079488No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2850.3250233788746208No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT2760.3147594826996328No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2760.3147594826996328No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2760.3147594826996328No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2730.31133818397463675No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2710.30905731815797277No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2380.27142303218301667No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2290.26115913600802865No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2280.26001870309969666No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2130.2429122094747166No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2070.2360696120247246No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1970.22466528294140456No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1850.21098008804142054No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1690.19273316150810849No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1660.18931186278311246No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1640.18703099696644848No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1560.17790753369979243No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1560.17790753369979243No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1540.17562666788312845No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1520.17334580206646444No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1500.17106493624980043No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1480.16878407043313642No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1480.16878407043313642No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1480.16878407043313642No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1440.1642223387998084No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1390.1585201742581484No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1380.1573797413498164No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1370.1562393084414844No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1360.15509887553315238No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1330.15167757680815638No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1330.15167757680815638No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1320.15053714389982437No Hit
NNNAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1280.14597541226649635No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGAAGACTTGGCCTCAGTTTCCC1260.14369454644983234No Hit
NNTAGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT1260.14369454644983234No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1230.14027324772483635No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1230.14027324772483635No Hit
NNTAGNGGCTGGGTGACTTGGTGAATTTGGCCTCAGTTTCCCCTCCTTCT1180.13457108318317634No Hit
NNNAGNGGCTGGGTGACTTGGTGAATTTGGCCTCAGTTTCCCCTCCTTCT1150.13114978445818032No Hit
NNNAGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT1140.13000935154984833No Hit
NNTAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1130.1288689186415163No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1130.1288689186415163No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1130.1288689186415163No Hit
NNNAGNTGGTGGGTTNTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1110.1265880528248523No Hit
NNTAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1100.12544761991652031No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1100.12544761991652031No Hit
NNTAGNCCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG1080.12316675409985631No Hit
NNTAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG1070.1220263211915243No Hit
NNNNGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1070.1220263211915243No Hit
NNNAGNCACACCCAANTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1020.11632415664986429No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1020.11632415664986429No Hit
NATAGATACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1010.11518372374153228No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1010.11518372374153228No Hit
NNNNGNGGCTGGGTGACTTGGTGAATTTGGCCTCAGTTTCCCCTCCTTCT1000.11404329083320028No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1000.11404329083320028No Hit
NNTAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT980.11176242501653628No Hit
NNNAGNCTGGCGGTGNTTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT980.11176242501653628No Hit
NNTNGNGGCTGGGTGACTTGGTGAATTTGGCCTCAGTTTCCCCTCCTTCT980.11176242501653628No Hit
NNNAGNCCTGACCTCNTGATCTGCCTGTGTTCTTTGCATGATCTATGTGT970.11062199210820428No Hit
NNTNGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT970.11062199210820428No Hit
NNNNGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG960.10948155919987226No Hit
NNNAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT960.10948155919987226No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGAAGACTTGGCCTCAGTTTCCC950.10834112629154026No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT940.10720069338320827No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT930.10606026047487627No Hit
NNNAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT930.10606026047487627No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT920.10491982756654426No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA910.10377939465821226No Hit
NNNAGNCACACCCAACTCTGCTCAGTTCTTTTTGTTTGGCAGACACACAC910.10377939465821226No Hit
NNNAGNCCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG900.10263896174988024No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC900.10263896174988024No Hit
NNNNGNCCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG900.10263896174988024No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG890.10149852884154827No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC890.10149852884154827No Hit
NATAGAGGCTGGGTGACTTGGTGAATTTGGCCTCAGTTTCCCCTCCTTCT890.10149852884154827No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCTACGGTAGCCTTGGCCTCAGTTTC880.10035809593321625No Hit
NNNNGNTTAGGGGTGACGGACTAGCCATGCTGAAGTCCCATGATCTATGT880.10035809593321625No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)