FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808351

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808351
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66226
Sequences flagged as poor quality0
Sequence length150
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNTAGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC9321.4073022679914233No Hit
NNNAGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC8811.3302932383051973No Hit
NNNNGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC8271.2487542656962523No Hit
NATAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC7901.1928849696493824No Hit
NNTAGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG7491.1309757497055537No Hit
NNNAGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG7331.106816054117718No Hit
NNTNGNTTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC6941.0479267961223688No Hit
NNNNGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG6570.9920575000754991No Hit
NATAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG6300.9512880137710265No Hit
NNTNGNCCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG5520.833509497780328No Hit
NNTAGNTGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG5330.8048198592697733No Hit
NNNAGNTGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG5270.7957599734243349No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4890.7383806964032253No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA4870.7353607344547459No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA4860.7338507534805061No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA4810.7263008486093075No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4500.6794914384078761No Hit
NNNNGNTGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG4500.6794914384078761No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4380.6613716667169993No Hit
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA4370.6598616857427597No Hit
NATAGATGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG4110.6206021804125268No Hit
NNTNGNTGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG3820.5768127321595748No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3820.5768127321595748No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA3690.5571829794944584No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3400.5133935312415063No Hit
NNTAGNCTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA3080.4650741400658352No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2990.45148431129767763No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2930.44242442545223926No Hit
NNNNGNCTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA2930.44242442545223926No Hit
NNTAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2890.43638450155528036No Hit
NNNAGNCTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA2810.42430465376136256No Hit
NNNAGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2730.4122248059674448No Hit
NNNNGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC2630.3971249962250476No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2600.39259505330232836No Hit
NNTNGNCTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA2590.3910850723280887No Hit
NNNNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2580.38957509135384893No Hit
NNTAGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2490.3759852625856914No Hit
NNNAGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2460.37145531966297224No Hit
NATAGACTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA2330.35182556699785583No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2320.3503155860236161No Hit
NNTAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2310.3488056050493764No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2290.34578564310089693No Hit
NNNNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA2270.3427656811524175No Hit
NNNAGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG2230.3367257572554586No Hit
NATAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT2220.33521577628121885No Hit
NNTAGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG2200.3321958143327394No Hit
NNNAGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA2140.32313592848730105No Hit
NNTAGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA2120.32011596653882163No Hit
NNNAGNTTGAGCTTANATGATCTGTGCAAGGTTAAACAATATCCAAATCC2110.31860598556458186No Hit
NNNNGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG2100.31709600459034215No Hit
NATAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA2100.31709600459034215No Hit
NNNAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC2090.31558602361610244No Hit
NNNNGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG2080.31407604264186273No Hit
NNTAGATTGAGCTTAAATGATCTGTGCAAGGTTAAACAATATCCAAATCC2060.31105608069338325No Hit
NNNAGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA2010.30350617582218464No Hit
NNTNGNTTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA1980.2989762328994655No Hit
NNTAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG1940.2929363090025066No Hit
NNNNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1930.29142632802826685No Hit
NNNAGNCCCTGGATTNGCATGAGGAACATGGCTTCCCATTTCAGACAAGG1930.29142632802826685No Hit
NNNNGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA1900.28689638510554766No Hit
NNTNGNGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1890.28538640413130795No Hit
NATAGAGGTGAGCGGCTGTCTCCATTCAACTTTCCTTGGCTCTATGTGTA1880.2838764231570682No Hit
NNTNGNTTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA1870.2823664421828285No Hit
NNTAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1840.2778364992601093No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1830.2763265182858696No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1810.27330655633739015No Hit
NATAGAATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA1790.27028659438891073No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1780.26877661341467096No Hit
NNTAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1760.26575665146619154No Hit
NATAGATATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG1740.26273668951771206No Hit
NNNAGACCCTGGATTAGCATGAGGAACATGGCTTCCCATTTCAGACAAGG1720.25971672756923264No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1700.25669676562075316No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1670.25216682269803403No Hit
NNNAGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1650.24914686074955453No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1640.24763687977531484No Hit
NNTNGNTATGCCCATGGTTAGGAAGGAAGGATCAATATTGTTAAAATGGG1640.24763687977531484No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1630.24612689880107508No Hit
NNNNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1620.2446169178268354No Hit
NNNAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1620.2446169178268354No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1550.2340470510071573No Hit
NNNNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1550.2340470510071573No Hit
NNNAGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1540.2325370700329176No Hit
NNTNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1530.23102708905867786No Hit
NNTNGNATAGCCAGCAGAAGGAAGCAACCCAAATGGACATTTCACTTATA1520.22951710808443815No Hit
NNTAGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1470.2219672032132395No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1460.2204572222389998No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1400.21139733639356145No Hit
NATAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTTGTATATCT1380.208377374445082No Hit
NNNNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1370.20686739347084226No Hit
NNTNGNCTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1370.20686739347084226No Hit
NNTNGNCCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1340.20233745054812308No Hit
NATAGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA1340.20233745054812308No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCACCTCTACT1320.19931748859964363No Hit
NNTAGATGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG1300.19629752665116418No Hit
NATAGAATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1260.1902576027542053No Hit
NNNAGATGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG1250.18874762177996557No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1210.18270769788300667No Hit
NNTNGNATCCTTTCCCCATTGCTTGCTCATTATTATTGGATGGGATCTAT1200.18119771690876693No Hit
NNNNGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1200.18119771690876693No Hit
NNNNGATGGAGAGAGTGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG1200.18119771690876693No Hit
NNNAGNTGGAGAGAGNGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG1180.17817775496028748No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1170.17666777398604777No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGATTACAGGCATGTGCCAC1170.17666777398604777No Hit
NNNNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1150.17364781203756832No Hit
NATAGACCAGACTTTTGAAAGAAGCACACAAAAAGGGCATCTATGTGTAG1150.17364781203756832No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1120.16911786911484916No Hit
NATAGATTGAGCTTANATGATCTGTGCAAGGTTAAACAATATCCAAATCC1120.16911786911484916No Hit
NNTAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1110.16760788814060942No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA1100.1660979071663697No Hit
NNTAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA1070.16156796424365052No Hit
NATAGAGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1060.16005798326941081No Hit
NNNAGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT1050.15854800229517108No Hit
NATAGACCCTGGATTNGCATGAGGAACATGGCTTCCCATTTCAGACAAGG1020.15401805937245192No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA990.14948811644973276No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG990.14948811644973276No Hit
NNNAGAATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA980.14797813547549302No Hit
NNTAGNTGAAAAGTCAAGCCAGCACAAATATTTGGAAACTAATTCCACTG980.14797813547549302No Hit
NNNNGNTGAAAAGTCAAGCCAGCACAAATATTTGGAAACTAATTCCACTG950.14344819255277383No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG930.14042823060429438No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT920.13891824963005464No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT900.13589828768157522No Hit
NNNAGNATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA900.13589828768157522No Hit
NATAGACTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG900.13589828768157522No Hit
NNNAGNTGAAAAGTCAAGCCAGCACAAATATTTGGAAACTAATTCCACTG860.12985836378461632No Hit
NNTAGNTGACTTGGGGGAAAATGGTATGATAGACACATAGATGACAGATA860.12985836378461632No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA860.12985836378461632No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT840.12683840183613687No Hit
NNNAGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACGCCAC830.12532842086189713No Hit
NATAGATGGAGAGAGNGAGGGAAAGGAAATTATGGGAGCCCTGTGAAACG820.12381843988765742No Hit
NATAGAATCCTTTCCNCATTGCTTGTTTTTCTCAGGTTTGTCAAAGATCA810.1223084589134177No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG800.12079847793917797No Hit
NNTNGNGGTGAGCGGCTGTCTCCAGATGATATGAACCCAAAGGTCTATGT800.12079847793917797No Hit
NNNAGNTGACTTGGGGGAAAATGGTAGAAACAGCCAGTTTTCCCTTCTAT800.12079847793917797No Hit
NNNNGACTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA790.11928849696493825No Hit
NNNAGNTGACTTGGGGGAAAATGGTATGATAGACACATAGATGACAGATA780.11777851599069852No Hit
NNTNGNCTGCCTCAGCCTCCTGAGTAGGTCAGGAGATTGAGACCATCCTG770.1162685350164588No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT760.11475855404221907No Hit
NATAGATGAAAAGTCAAGCCAGCACAAATATTTGGAAACTAATTCCACTG760.11475855404221907No Hit
NNNAGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG760.11475855404221907No Hit
NNNNGNTGACTTGGGGGAAAATGGTATGATAGACACATAGATGACAGATA760.11475855404221907No Hit
NNNNGNATCCTTTCCCCATTGCTTGTTCATTATTATTGGATGGGATCTAT750.11324857306797935No Hit
NNTAGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA750.11324857306797935No Hit
NNNAGNTTGCATAGCNATGTGTAGCCCCTAAAGCCCTTGTGAAGTTCTAT750.11324857306797935No Hit
NNTAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG750.11324857306797935No Hit
NNNAGACTTCCTTGCCCCCATTTATTGCTGAAACATTTTTCTGCATGCAA720.10871863014526018No Hit
NNTNGNTGAAAAGTCAAGCCAGCACAAATATTTGGAAACTAATTCCACTG720.10871863014526018No Hit
NNNNGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACGCCAC710.10720864917102045No Hit
NATAGAATCCTTTCCCCATTGCTTGTATACATCTATGTGTAGAACCATGT710.10720864917102045No Hit
NNNAGNCTTCCTTGCNCCCATTTATTGCTGAAACATTTTTCTGCATGCAA700.10569866819678073No Hit
NNNAGNATCCTTTCCCCATTGCTTGTTTTTCTCAGGTTTGTCAAAGAAAG690.104188687222541No Hit
NNNNGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA690.104188687222541No Hit
NNTAGNCTGCCTCAGCCTCCTGAGTAGCTGGGACTACAGGTGCACGCCAC690.104188687222541No Hit
NNTAGATTGCATAGCCATGTATAGCCCCTAAGCCCAGGTCTATGTGTAGA680.10267870624830126No Hit
NNTAGNTTGAGCTTAAATGATCTGTGCAACCCCTTAAGAAAATTTAATTG680.10267870624830126No Hit
NNNNGATTGCATAGCCATGTGTAGCCCCTAAGCCCAGGTCTATGTGTAGA680.10267870624830126No Hit
NNTAGNAGTTCTGATCCACATTCACGTATAGCCCCTAAGCCCAGGTCTAT670.10116872527406154No Hit
NNNNGACTGTCATTGCTTGCCCATTGCTTGCTCATTATTATTGGATGGGA670.10116872527406154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)