FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110961
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA57325.165778967384937No Hit
NNTAGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA57045.140544876127649No Hit
NNNNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA56175.062138949721073No Hit
NATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA54014.86747596002199No Hit
NNTNGNGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA44063.9707645028433416No Hit
NNNNGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA16061.4473553771144816No Hit
NNTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14791.33290074891178No Hit
NNNAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14601.3157776155586196No Hit
NNNAGNGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA14401.2977532646605565No Hit
NATAGAGGTGAGCGGNTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA9190.8282189237660078No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG9010.8119970079577509No Hit
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8950.806589702688332No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA8110.7308874289164662No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA7850.7074557727489839No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG7610.6858265516713079No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA7460.6723082884977605No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG6840.6164328007137643No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6830.6155315831688611No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG6770.6101242778994421No Hit
NATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6600.5948035796360883No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6480.5839889690972504No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA6250.5632609655644776No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6130.5524463550256397No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5030.4533124250862916No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG4980.4488063373617758No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG4870.438892944367841No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4840.4361892917331314No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4500.40554789520642387No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4480.4037454601166175No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4290.3866223267634574No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT4100.3694991934102973No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4000.3604870179612657No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3910.35237606005713723No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT3890.35057362496733085No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3560.32083344598552643No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT3510.3163273582610106No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3480.3136237056263011No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3410.307315182811979No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3360.3028090950874631No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3240.2919944845486252No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3180.2865871792792062No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3150.28388352664449673No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3140.28298230909959354No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2960.2667603932913366No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2910.2622543055668208No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2710.24422995466875747No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2620.236118996764629No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG2440.21989708095637206No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2350.21178612305224356No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG2330.20998368796243724No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2300.20728003532772776No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2280.20547760023792144No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2180.1964654247888898No Hit
NNTAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA2160.19466298969908347No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2100.18925568442966448No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2040.1838483791602455No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2010.181144726525536No Hit
NNNAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1960.17663863880102018No Hit
NNNNGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1950.17573742125611702No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1940.17483620371121386No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1940.17483620371121386No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1830.16492281071727904No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1810.16312037562747272No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1720.15500941772334426No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1680.15140454754373156No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1660.14960211245392527No Hit
NNNAGNCCAGCTTTGNATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA1630.14689845981921576No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA1610.14509602472940944No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1570.14149115454979677No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1540.13878750191508726No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1490.13428141419057146No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1410.12707167383134615No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1410.12707167383134615No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1380.12436802119663666No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1300.11715828083741135No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1280.11535584574760502No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1270.11445462820270184No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1220.10994854047818603No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1200.10814610538837971No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1200.10814610538837971No Hit
NNNNGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1190.10724488784347654No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1190.10724488784347654No Hit
NNNAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1150.10364001766386388No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1150.10364001766386388No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1120.10093636502915437No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)