FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808435

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808435
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40603
Sequences flagged as poor quality0
Sequence length150
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGGGCCTGGTTATTACTTGGAGGCTGGAGAAAAAGCTCTATGTG416010.245548358495677No Hit
CATAGATTCTTTCCTGGCTGTGGGTGGGTTGAGGGAGTCTTCTCTATGTG36679.031352363125878No Hit
CATAGAAGGAACAGCAGAGAGGGCAGGCCCTTCAGCATCATTTCTATGTG35198.666847277294782No Hit
CATAGAAGTATTCACACTGGGGCCCAGAACCCATTGTAGATTTCTTTCAC24436.016796788414649No Hit
CATAGAGCTCATTCAACTAATAACTGGTAGACCATGGAGAGAATAATCCG17904.408541240795015No Hit
CATAGAATCTGGTTCAAGCACACAGTTGTGGAAAGTGCAATGAGTCTATG15743.87656084525774No Hit
CATAGATGGGACAGCGACAATGACAGTTGTGGAAAGTGCAATGAGTCTAT14293.519444376031328No Hit
CATAGAGCTAATAAAGACATACCTGAGCCATGCTGAAGTCCCATGATCTA13013.2041967342314606No Hit
CATAGATCTCACAGAAACTTCCTTGATGAGCATGCCTTTGATAAAACATC9072.2338250868162453No Hit
CATAGATCTAGCCCATCACTTCAGCAGGCCCTTCAGCATCATTTCTATGT7621.8767086175898333No Hit
CATAGACCAGTACGCCTTACCCCTTGGAGGCTGGAGAAAAAGCTCTATGT7081.7437135187055146No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACAGTTGTGGAAAGTGCAATGAG6761.6649016082555474No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGTAGAACCATGT6241.5368322537743517No Hit
CATAGATCTCACAGAAACTTCCTTGATGATGGGAGTGTCTGCTGGTCTAT4481.1033667462995345No Hit
CATAGACCTCCCATGGTTCCTGATGAGCATGCCTTTGATAAAACATCTAT4060.9999261138339531No Hit
CATAGATCTAGCCCATCACTTCAGCAGCAGGCCCTTCAGCATCATTTCTA3770.9285028199886709No Hit
CATAGACCCTCTCTTACCCTTCCACCAACATGCTTTTAGTCACGTCTATG3450.8496909095387041No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTACAGTTGTGGAAAGTGC3440.8472280373371426No Hit
CATAGAACACGAAGATTTCAGATTAGCCTCTCCAGTTTCTACGTGATCTA3350.8250621875230895No Hit
CATAGAAGTATTCACACTGGGGCCCATTGTAGATTTCTTTCACCTCCTCT3220.7930448489027905No Hit
CATAGACCTCCCATGGTTCCTGATGATGGGAGTGTCTGCTGGTCTATGTG3090.7610275102824914No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTATAGATGCCTGGCCAACTGTTTT2710.6674383666231559No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA2530.6231066669950497No Hit
CATAGAGGCTGCAGTGAACAATGATCGTCAGTGTGGGCTGGTGTTTTCCT2520.6206437947934882No Hit
CATAGAAGGAACAGCAGAGAGGGCAGTGTGGGTCCTAAATGTCTTTGTCC2510.6181809225919267No Hit
CATAGATTCAGTTTACTTAGAGGCAGACAGTTGTGGAAAGTGCAATGAGT2450.603403689382558No Hit
CATAGACCCTCTCTTACCCTTCCACCTGAATGATGGGAGTGTCTGCTGGT2250.5541462453513287No Hit
CATAGATGAAGGCAGAAAAGGAAGAACAACAACAAAACTCGTGACTTCTA2170.5344432677388371No Hit
CATAGACCCCATGACTCAGCTCCTCATTCTAAAAAAGAACGATGAAATAC2010.4950373125138537No Hit
CATAGATTCCTCCAAAGACTGCAGGGTCCTAAATGTCTTTGTCCTTCTAT1980.48764869590916926No Hit
CATAGACCAGTTACCACAAGAAGCAATGTCAGACCCAGGCTACACATCTA1690.41622540206388686No Hit
CATAGAAGCATCCAGGTTTCTCATTTGTCAGACCCAGGCTACACATCTAT1610.39652242445139524No Hit
CATAGACCAGTACGCCTTACCCCTTGGGCTGGTGTTTTCCTTGATCTATG1580.38913380784671087No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGAGCATGCCTTTGATAAAAC1580.38913380784671087No Hit
CATAGATGGCAAATGGTGGGATCTCTATAGATGCCTGGCCAACTGTTTTT1550.3817451912420265No Hit
CATAGATTCTTTCCTGGCTGTGGGTGCATAAAAACTGTCGGAGGTCTATG1540.379282319040465No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGGTTGGAGGCTGGAGAAAAAG1520.37435657463734207No Hit
CATAGACCCTCTCTTACCCTTCCACCCTGGGAGAAGAAATGTTGGTCTAT1520.37435657463734207No Hit
CATAGATGGCCCAGAATCCTTTGTAGAACCAATGTGCTCGTATCTTGGCC1460.35957934142797326No Hit
CATAGAGGCTGCAGTGAACAATGATCGTCAGTGTATGATGGGAGTGTCTG1400.3448021082186045No Hit
CATAGATGACATGGGAAATTATGCTGCTATGGTGGGTTGAGGGAGTCTTC1350.33248774721079727No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGCAGGCCCTTCAGC1240.30539615299362116No Hit
CATAGAATCTGGTTCAAGCACACAGTGGGCTGGTGTTTTCCTTGATCTAT1230.3029332807920597No Hit
CATAGAAATTTTTCAGAGACAGTTGGGACAGTGCATAAAAACTGTCGGAG1140.28076743097800655No Hit
CATAGAGGCTTCCAGTCCTTTAAAATAAATGATGGGAGTGTCTGCTGGTC1060.26106445336551487No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGCAGGCCCTTCA1050.2586015811639534No Hit
CATAGATGGCCCAGAATCCTTTGTAGATTTCTTTCACTCTCCCACCATTC1050.2586015811639534No Hit
CATAGATTCTTTCCTGGCTGTGGGTGGGCTGGTGTTTTCCTTGATCTATG1050.2586015811639534No Hit
CATAGAAGGAACAGCAGAGAGGGCAGTGTTGTTACAAAAATTGGGAAGCG1040.25613870896239194No Hit
CATAGAGCAGCTTGAATTCTCCTCAGACCATGGAGAGAATAATCCGTCTA980.2413614757530232No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCGCAGGCCCTTCAGCATC960.23643573134990026No Hit
CATAGAAGCATCCAGGTTTCTCATTTGTGACTCACTCTCCCACCATTCAG920.22658424254365442No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCTATGTGTAGAACCATG900.22165849814053148No Hit
CATAGAGGCTGCAGTGAACAATGATCGTCAGTGTACCCTGGGAGAAGAAA850.20934413713272418No Hit
CATAGAGTGTGTAGTGTGGTATAGCGTCAGTGTAGCCCGTTCTCTCTGTT830.20441839272960127No Hit
CATAGATTCAGAGAAGGACAGCGTGGGCTGGTGTTTTCCTTGATCTATGT820.20195552052803978No Hit
CATAGACCAGTTACCACAAGAAGCAATGGCCTGGGTCCTAAATGTCTTTG810.19949264832647834No Hit
CATAGACCAGTTACCACAAGAAGCAATGAGCATGCCTTTGATAAAACATC800.1970297761249169No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTCTATTAACACGCACAAA800.1970297761249169No Hit
CATAGAGGGGTAATACTTCCTACTTCTGCAGGCCCTTCAGCATCATTTCT790.19456690392335543No Hit
CATAGAGCTAATAAAGACATACCTGAGCCATGTCGACTGTTAATATAGAA760.18717828731867103No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTGCAGGCCCTTCAGCATCAT720.1773267985124252No Hit
CATAGATGGCAAATGGTGGGATCTCTATGTGTAGAACCATGTCGTCAGTG710.17486392631086373No Hit
CATAGATCTAGCCCATCACTTCAGCATCAGCAGGCCCTTCAGCATCATTT700.17240105410930226No Hit
CATAGAGGTGAGCGGCTGTCTCCAGTTTCTACGTGATCTATGGTAGAACC690.16993818190774082No Hit
CATAGACCAGTTACCACAAGAAGCAATGTGGTGGGTTGAGGGAGTCTTCT660.16254956530305642No Hit
CATAGATTCAGAGAAGGACAGCGTGGTGCTGGAATAGTTGGACTCTATGT650.16008669310149495No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACACTACGAATGAGGAAACAGG640.1576238208999335No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGGCAGGCCCTTCAG610.15023520429524911No Hit
CATAGACCTCCCATGGTTCCTGATGAAATGATGGGAGTGTCTGCTGGTCT600.14777233209368765No Hit
CATAGATGACATGGGAAATTATGCTGCTCGCAGGCCCTTCAGCATCATTT570.14038371548900327No Hit
CATAGATCTCACAGAAACTTCCTTGATGTTCGCACCCAAGCTCTATGTGT550.13545797108588034No Hit
CATAGATCTAGCCCATCACTTCAGCATCCAGGCCCTTCAGCATCATTTCT540.13299509888431887No Hit
CATAGAGGGGTAATACTTCCTACTTCTGCATTAGGAAGTCTTCTCAGCTT540.13299509888431887No Hit
CATAGAACTTTTTCAGAGACAGTTGGGACAGTTGTGGAAAGTGCAATGAG540.13299509888431887No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGTAGAACCATGG510.1256064822796345No Hit
CATAGAACACGAAGATTTCAGATTAGCCTTCCTTTCAATAGCCTTTTAGG500.12314361007807306No Hit
CATAGAGGCTGCAGTGAACAATGATCGTCAGTGTATGGGTCATTGTGAGG500.12314361007807306No Hit
CATAGATCTAGCCCATCACTTCAGCATCATTTCTATGTGTAGAGCAGGCC450.11082924907026574No Hit
CATAGATTCTTTCCTGGCTGTGGGTGGGTTGATGTTCGCACCCAAGCTCT430.10590350466714282No Hit
CATAGAAGGAAGTAGGGCATGACAAAAATGTAAAGGCTGGGGTCTTGGGT430.10590350466714282No Hit
CATAGATGGTATTTGAGTGGGGAAACAGACGAATGAGGAAACAGGCACTC420.10344063246558136No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)