FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences92559
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA3894542.07586512386694No Hit
CATAGACCAGCTTTGCATGAGTGTGGTTAATGAAGCTCTTCCATGTCCCA33003.5652934884776193No Hit
CATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG32763.5393640812886913No Hit
CATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG24102.603744638554868No Hit
CATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT16871.8226212469884076No Hit
CATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG13391.4466448427489493No Hit
CATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC12651.3666958372497542No Hit
CATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG11451.2370488013051135No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTTACGGTAGCAAAGCT7760.8383841657753432No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA6570.7098175217969079No Hit
CATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG5970.6449940038245876No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTACGGTAGCAAAGCTG5850.6320293002301235No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTTAATCTATGTGTAGAACCAT4950.5347940232716429No Hit
CATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG4260.46024697760347455No Hit
CATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3770.4073077712594129No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGCAGAGACTTGGTCTCATAGAG3750.4051469873270023No Hit
CATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC3280.3543685649153513No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGCAAAGC2920.31547445413195907No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTGAAGTCCCCATCAATTTA2910.3143940621657538No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT2840.30683131840231637No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCAGAGACTTGGCCTCAGTTTCCCC2820.30467053446990566No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACTTGGTCTCATAGAGGGGAG2790.30142935857128966No Hit
CATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT2770.299268574638879No Hit
CATAGAGTGGGGGACTAGCATTGATTCTATGTGTAGAACCATGTCGTCAG2730.2949470067740576No Hit
CATAGATACGATCATAAGGGTGGGGCTGGGCTCAGTGGCTTATGCCTGTA2690.29062543890923626No Hit
CATAGACTTACTGCCCTCACTCCGTCTGCATAGCCACATCTATGTGTAGA2650.2863038710444149No Hit
CATAGATGACCTGTTAATCTGCCCCCGTACTTTCTGGGTAGCTGCGACCT2570.2776607353147722No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGACACTGGGGGCACAAAGTTCT2520.2722587754837455No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATAGGCAAAGCTGCT2460.26577642368651344No Hit
CATAGAGGGGAGACACTGGGGGCACAAAGTTCTATGTGTAGAACCATGTC2410.2603744638554868No Hit
CAGTTTCCCCTCCTTCTATGTGTAGAACCATGTCGTCAGTGTAGATCTCG2280.24632936829481739No Hit
CATAGATGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC2260.24416858436240668No Hit
CATAGACCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA2240.242007800429996No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCTTGGCCTCAGTTTCCCCTCCTTC2240.242007800429996No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTAGGAGACTCTGG2220.2398470164975853No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTGGCCTCAGTTTCCCCTCCTTCTA2160.23336466470035327No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCG2070.22364113700450525No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTACCAGTGTAGCAGA2030.2193195691396839No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA2010.2171587852072732No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCGGGTCA1970.21283721734245187No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCCGCCTCCCAAAGTGCTGGG1960.2117568253762465No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTCAGCCTCCCAAAGTGCTGGG1920.20743525751142516No Hit
CATAGAGGTGAGCGGCTGTCTCCAAGTCCGCGCAGAGCCTTCAGGTCAGA1900.20527447357901446No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCCTCAGGTCA1900.20527447357901446No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGACTTGGTCTCATAGAGGGG1890.2041940816128091No Hit
CATAGATGATGTGAAAAGGACCCATGGCTGAGAGAAAACTACCCCAGGCT1840.19879212178178243No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTGAGACTTGGCCTCAGTTTCCCCT1840.19879212178178243No Hit
CATAGAGGTGAGCGGCTGTCTCCACGAGTCCGCGCAGAGCCTTCAGGTCA1750.18906859408593438No Hit
CATAGACCAGCTTTGCATGAGTGTGGAACCATACCAGTGTAGCAGACCAT1700.18366663425490767No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCGGAGCCTTCAGGTCA1690.18258624228870235No Hit
CATAGACCTGACCTCATGATCTGCCTGCCTTGGCCTCCCAAAGTGCTGGG1670.18042545835629167No Hit
CATAGAGGGGAGACACTGGCTATAAGAAAGACACTGGGGGCACAAAGTTC1670.18042545835629167No Hit
CATAGAGGTGAGCGGCTGTCTCCAGAAGTCCGCGCAGAGCCTTCAGGTCA1630.1761038904914703No Hit
CATAGACCTCCTTTCCTCCACGGCAAAGCTGCTATGGGATCTTCTATGTG1600.1728627145928543No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGAGGGGAGACACTGGGGGCACA1560.16854114672803294No Hit
CATAGATGACCTGTTAATCTGCCCCCGTAGCAAAGCTGCTATGGGATCTT1550.1674607547618276No Hit
CATAGATGATGTGAAAAGGACCCATGGAGAACCAGTGTAGCAGACCATCT1530.16529997082941691No Hit
CATAGACACACCCAACTCTGCTCAGTGTACCAGTGTAGCAGACCATCTTC1520.16421957886321156No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTCCAGGTCA1490.16097840296459556No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTCGGTAGCAAAGCTGC1480.15989801099839024No Hit
CATAGAGGGGAGACACTGGCTATAAGAATAGAGGGGAGACACTGGGGGCA1470.1588176190321849No Hit
CATAGAGGTGAGCGGCTGTCTCCACAGGTCCGCGCAGAGCCTTCAGGTCA1470.1588176190321849No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGCCA1370.14801369937013148No Hit
CATAGACCTGACCTCATGATCTGCCTACCTCAGCCTCCCAGATTGCTGGG1360.14693330740392616No Hit
CATAGACCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG1350.1458529154377208No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGGAGACACTGGGGGCACAAA1340.14477252347151545No Hit
CATAGACTTACTGCCCTCACTCCGTCTACGGTAGCAAACACTCAAAACAG1340.14477252347151545No Hit
CATAGATGTGAGCACTACTGATAATCCCTGACTTGGCCTCAGTTTCCCCT1330.14369213150531013No Hit
CATAGATGACCTGTTAATCTGCCCCCGTATGATGTGGCATGGAGAAGGGT1320.14261173953910478No Hit
CATAGACTTACTGCCCTCACTCAGTCTGCATAGCCACATCTATGTGTAGA1310.14153134757289945No Hit
CATAGATGATGTGAAAAGGACCCATGGGATGATGTTTTAAAATGAGCACC1300.14045095560669413No Hit
CATAGATGATGTGAAAAGGACCCATGGCGTCAGTGTGCACACTACAATTA1180.12748625201223004No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCTTTCAGGTCA1160.12532546807981934No Hit
CATAGAGTCTCAATTATTCATCTCAACATGCTGAGGGGAGACACTGGGGG1140.12316468414740869No Hit
CATAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGGGCCTTCAGGTCA1130.12208429218120334No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCTCATAGATTTATCCAGCATGCCCC1120.121003900214998No Hit
CATAGAGATGATGGGATCTTATATTTGGAAAAACTATTTGAGGTAAAACT1070.11560194038397131No Hit
CATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1020.11019998055294461No Hit
CATAGAGGGGAGACACTGGCTATAAGAAGGGAGACACTGGGGGCACAAAG980.10587841268812324No Hit
CATAGACTTTTCCTCCAATATAGCTGCACTACTTGGCCTCAGTTTCCCCT930.10047645285709657No Hit
CGTAGAGGTGAGCGGCTGTCTCCACAAGTCCGCGCAGAGCCTTCAGGTCA930.10047645285709657No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)