FastQCFastQC Report
Wed 28 Jun 2023
EGAF00005808499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005808499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80373
Sequences flagged as poor quality0
Sequence length150
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11241.3984795889166761No Hit
NNTAGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG11181.3910143953815335No Hit
NNNNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG10161.2641061052841127No Hit
NNTAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8751.0886740572082665No Hit
NNNAGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG8561.0650342776803154No Hit
NATAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8401.0451270949199358No Hit
NNTNGNCCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG8301.0326851056946984No Hit
NNNNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG7340.91324200913242No Hit
NNTNGNTACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6950.8647182511539946No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT6780.843566869471091No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT6700.8336132780909012No Hit
NNTAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6200.7714033319647146No Hit
NNNAGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT6190.7701591330421907No Hit
NATAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG6050.7527403481268585No Hit
NNNNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT5720.7116817836835754No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5380.6693790203177684No Hit
NATAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5070.6308088537195328No Hit
NNTNGNGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT5060.629564654797009No Hit
NNNAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4950.6158784666492478No Hit
NATAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4910.6109016709591529No Hit
NNTNGNCACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT4700.5847734935861546No Hit
NNTAGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4700.5847734935861546No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4650.5785524989735359No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4600.5723315043609173No Hit
NNTAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4450.5536685205230613No Hit
NNNNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4390.5462033269879188No Hit
NNNAGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG4320.5374939345302526No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC4200.5225635474599679No Hit
NNNAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4200.5225635474599679No Hit
NNTAGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG4190.5213193485374442No Hit
NATAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT4160.517586751769873No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT4110.5113657571572543No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3810.47403978948154235No Hit
NNNNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3780.4703071927139711No Hit
NATAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3710.4615978002563049No Hit
NNNNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3670.45662100456621No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3640.45288840779863887No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC3630.4516442088761151No Hit
NNTAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3550.44169061749592525No Hit
NATAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3490.43422542396078284No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT3460.43049282719321164No Hit
NNTNGNCCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT3450.42924862827068794No Hit
NNNAGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3270.4068530476652607No Hit
NNNNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT3240.40312045089768955No Hit
NNTNGNTGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG3210.3993878541301183No Hit
NNTNGNCACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG3050.37948067136973856No Hit
NNTAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA3030.3769922735246911No Hit
NNNAGNCCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG3000.3732596767571199No Hit
NNNNGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2960.368282881067025No Hit
NATAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2950.36703868214450125No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC2920.36330608537693004No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2840.3533524939967402No Hit
NNNAGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2820.35086409615169273No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2750.3421547036940266No Hit
NNNAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2690.3346895101588842No Hit
NATAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2660.330956913391313No Hit
NNTAGACCTCCTTTCCTCCACGGCAAACACTCAAAACAGCCCTCTATGTG2590.32224752093364684No Hit
NNNNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2530.3147823273985045No Hit
NNTNGNTGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT2410.2998519403282197No Hit
NNTNGNGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2260.2811889564903637No Hit
NATAGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2230.2774563597227925No Hit
NNNNGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG2230.2774563597227925No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG2190.2724795640326975No Hit
NATAGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA2180.2712353651101738No Hit
NNTAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1960.24386298881465168No Hit
NNNNGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1920.23888619312455675No Hit
NNTNGNCCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG1900.2363977952795093No Hit
NNNAGATACGATCATAAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1890.23515359635698554No Hit
NNNAGNTACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG1860.23142099958941434No Hit
NNNNGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1730.21524641359660582No Hit
NNNAGNCACCATTGGNGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1690.2102696179065109No Hit
NNNAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1680.20902541898398713No Hit
NNNAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1660.20653702113893968No Hit
NNTAGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1560.19409503191370236No Hit
NNNNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1540.19160663406865489No Hit
NNNAGNGTTTGCCCTNCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1540.19160663406865489No Hit
NNTAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1530.19036243514613116No Hit
NATAGACACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1500.18662983837855995No Hit
NNNAGACACCATTGGAGACCTGAGCATCCCTCATTACTCTGGTTCTATGT1490.18538563945603623No Hit
NNNNGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1480.1841414405335125No Hit
NNNAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1430.17792044592089384No Hit
NNNNGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1350.16796685454070398No Hit
NNTAGAGTTTGCCCTTCCTTCCCTCCCGGATTCAAGCAATTCTTCTATGT1350.16796685454070398No Hit
NNTAGACCTGACCTCATGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1320.16423425777313275No Hit
NATAGATGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1270.1580132631605141No Hit
NNTNGNCACACCCAACTCTACTCAGTCTGCATAGCCACATCTATGTGTAG1270.1580132631605141No Hit
NNNAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1260.15676906423799036No Hit
NATAGACCTCCTTTCNTCCACGGCAAACACTCAAAACAGCCCTCTATGTG1260.15676906423799036No Hit
NNNNGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1250.15552486531546664No Hit
NNNNGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1190.14805967178032425No Hit
NNNAGNCCTGACCTCNTGATCTGCCTGTGTTCTTTGCATGATCTATGTGT1170.14557127393527677No Hit
NNNAGNCTGGCGGTGNTTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1170.14557127393527677No Hit
NNTAGNGTCTCAATTATTCATCTCAACATGCTACTTGGCCTCAGTTTCCC1170.14557127393527677No Hit
NNNAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1160.14432707501275305No Hit
NNNAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC1140.14183867716770557No Hit
NNNAGNGTCTCAATTATTCATCTCAACATGCTACTTGGCCTCAGTTTCCC1120.1393502793226581No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC1090.1356176825550869No Hit
NNTAGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC1070.13312928471003943No Hit
NNNNGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT1070.13312928471003943No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT1060.1318850857875157No Hit
NNNAGNTGGTGGGTTNTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1030.1281524890199445No Hit
NNTAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1010.12566409117489705No Hit
NNNNGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1010.12566409117489705No Hit
NNNAGATGGTGGGTTCTGGAGAGAGCATCCCTCATTACTCTGGTTCTATG1010.12566409117489705No Hit
NNNNGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT1010.12566409117489705No Hit
NNNAGNCACACCCAANTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG1000.12441989225237332No Hit
NNNAGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA1000.12441989225237332No Hit
NNNAGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC980.12193149440732584No Hit
NNTNGNTGTGAGCACTACTGATAATCCCTGATGTGGCATGGAGAAGGGTC980.12193149440732584No Hit
NNTAGNGATGATGGGATCTTATATTTGGAAAAACTATTAGCGAGACTCTA970.1206872954848021No Hit
NNTAGNCTGGCGGTGATTGCTTTCCTACTTGGCCTCAGTTTCCCCTCCTT970.1206872954848021No Hit
NNNAGNCCAGCTTTGNATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC960.11944309656227838No Hit
NNNNGNGTCTCAATTATTCATCTCAACATGCTACTTGGCCTCAGTTTCCC960.11944309656227838No Hit
NNTAGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG950.11819889763975465No Hit
NNTNGNGTCTCAATTATTCATCTCAACATGCTACTTGGCCTCAGTTTCCC940.11695469871723092No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTACTTGGCCTCAGTTTCCCCTCCTT940.11695469871723092No Hit
NNNNGACACACCCAACTCTGCTCAGTCTGCATAGCCACATCTATGTGTAG930.11571049979470717No Hit
NNNAGATGTTTGAAACTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT930.11571049979470717No Hit
NNTAGNCCAGCTTTGCATGAGTGTGGCATGGAGAAGGGTCTATGTGTAGA920.11446630087218344No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC920.11446630087218344No Hit
NNTAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG920.11446630087218344No Hit
NATAGATACGATCATNAGGGTGGGGCGAAAGTGATCAGAAGCTCTATGTG900.11197790302713599No Hit
NATAGAGTCTCAATTATTCATCTCAACATGCTACTTGGCCTCAGTTTCCC900.11197790302713599No Hit
NNTNGNCTGGCGGTGATTGCTTTCCTACTTGGCCTCAGTTTCCCCTCCTT900.11197790302713599No Hit
NNTAGACCAGCTTTGCATGAGTGTGGGAAGTAGGGAATGATTACAAGTTC890.11073370410461224No Hit
NNTAGACTGGCGGTGATTGCTTTCCTCCCGGATTCAAGCAATTCTTCTAT890.11073370410461224No Hit
NNNNGNCTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC890.11073370410461224No Hit
NNNAGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT880.10948950518208851No Hit
NNNAGNTGTTTGAAANTCCTCTACCTAAGACACTGGGGGCACAAAGTTCT880.10948950518208851No Hit
NNTAGNGTTTGCCCTTCCTTCCCTCCAAGCAGATCTGTGTTCTATGTGTA870.10824530625956477No Hit
NNNNGNGTTTGCCCTTCCTTCCCTCCACGGCAAACACTCAAAACAGCCCT860.10700110733704105No Hit
NNNAGNCTGGCGGTGATTGCTTTCCTACTTGGCCTCAGTTTCCCCTCCTT850.10575690841451732No Hit
NNNAGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG840.10451270949199357No Hit
NNTAGNAAGGCAATGTTCAAGTGGTTTTGACTTGGCCTCAGTTTCCCCTC840.10451270949199357No Hit
NNNNGACCAGCTTTGCATGAGTGTGGCCTGTGTTCTTTGCATGATCTATG830.10326851056946984No Hit
NATAGACTGGCGGTGATTGCTTTCCTCCACGGCAAACACTCAAAACAGCC820.10202431164694611No Hit
NNTNGNCCAAACACAGAAGACATGGCAAACACTCAAAACAGCCCTCTATG820.10202431164694611No Hit
NNNAGNAAGGCAATGTTCAAGTGGTTTTGACTTGGCCTCAGTTTCCCCTC810.10078011272442239No Hit
NNNNGAGTTATTCAGAGAGAGGGGAAATGAATGTTGGAGAGGCACATCTA810.10078011272442239No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)