FastQCFastQC Report
Tue 31 May 2022
EGAF00005812735

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005812735
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000000
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGAGATTA1892480.378496No Hit
GTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACT1855760.371152No Hit
CCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGG1473010.29460200000000003No Hit
GGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTA1420710.284142No Hit
GCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGAGA1380950.27619No Hit
GGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAAC1329820.265964No Hit
GGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACT1286920.257384No Hit
ATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCC1267190.253438No Hit
GATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG1251280.250256No Hit
GAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTT1224700.24494No Hit
GCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCCCGGCAAGTG1209270.24185399999999999No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGT1185850.23717No Hit
GTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC1151210.230242No Hit
GGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCT1082590.21651800000000002No Hit
GTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGT1068310.21366200000000002No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC1062090.212418No Hit
GTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTT1056260.21125200000000002No Hit
GGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGA1019820.203964No Hit
GCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCCCGGCAA980470.196094No Hit
AGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAC908620.181724No Hit
CTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAA906850.18137No Hit
GGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGA891890.178378No Hit
GCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCC888960.177792No Hit
GTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTG888310.17766200000000001No Hit
CTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGA883440.17668799999999998No Hit
AAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGA861590.172318No Hit
ATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGG847470.169494No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG818310.163662No Hit
GATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACC784900.15698No Hit
CAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCC765470.153094No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA761960.152392No Hit
AATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTC742940.148588No Hit
CCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGA727660.145532No Hit
AGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTT725120.145024No Hit
GATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGC723660.144732No Hit
CACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCA721020.144204No Hit
AGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCA715250.14304999999999998No Hit
CAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCA706450.14129No Hit
GGAGGCATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGAT699030.139806No Hit
CTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCC691420.138284No Hit
AGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACAC687720.137544No Hit
GAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAA682170.136434No Hit
CTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGA674990.134998No Hit
CTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGA674280.134856No Hit
CCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATC644710.128942No Hit
CAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTG634920.12698399999999999No Hit
GAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGAGATTAGGA633900.12678No Hit
GGCATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACC630430.126086No Hit
CATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCA618670.123734No Hit
GAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCC617510.12350200000000001No Hit
AGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATT617130.12342600000000001No Hit
CCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGC601460.120292No Hit
GCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTG598950.11979000000000001No Hit
ATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAC590560.118112No Hit
CTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCT583130.116626No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA574160.114832No Hit
ACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAG564160.11283199999999999No Hit
AGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCC557370.11147399999999999No Hit
AAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAG549930.109986No Hit
ATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCA534610.106922No Hit
CAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAG519850.10396999999999999No Hit
AGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTG517420.10348399999999999No Hit
CTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGC513730.102746No Hit
GCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG507410.101482No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)