FastQCFastQC Report
Sat 28 May 2022
EGAF00005812835

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005812835
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37484796
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGAGATTA1485340.3962513228029839No Hit
GTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACT1477730.3942211663630236No Hit
CCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGG1174350.31328701908901946No Hit
GGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTA1128600.30108207071475057No Hit
GCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGAGA1084030.2891919166373481No Hit
GGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAAC1054470.2813060527260172No Hit
GGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACT1023810.27312673650404823No Hit
ATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCC1005400.26821541192327686No Hit
GATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATG991010.264376522150474No Hit
GAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTT962220.25669607485658985No Hit
GTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGT946510.2525050423110212No Hit
GCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCCCGGCAAGTG945660.2522782837073463No Hit
GTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGAC916980.24462718164452593No Hit
GGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCT848060.22624106050890608No Hit
GTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGT844400.22526466463896455No Hit
GGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCC841450.2244776788967986No Hit
GTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTT835430.2228716944331243No Hit
GGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGA820840.2189794496947509No Hit
GCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCCCGGCAA770570.20556867909858706No Hit
AGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCAC719340.19190180466768447No Hit
CTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAA715780.19095208628052823No Hit
GGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGA712660.1901197488176273No Hit
GTTGGCAGTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTG711920.18992233544501616No Hit
GCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCC710440.1895275086997939No Hit
CTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGA701090.18703316405936957No Hit
AAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGA688500.18367446897670192No Hit
ATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGG663570.17702377251832985No Hit
GCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTG654310.17455343761241224No Hit
GATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACC624180.16651551204920523No Hit
GAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCA603120.16089723417462376No Hit
CAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCC595800.15894444243474073No Hit
AATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTC586340.15642075256325258No Hit
CCAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGA583820.15574847999706334No Hit
GATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGC577530.1540704663298688No Hit
AGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTT571530.15246981736275156No Hit
CACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCA571460.15245114312480185No Hit
AGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCA567550.1514080535478971No Hit
CAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCA563690.15037830271238503No Hit
GGAGGCATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGAT553400.147633189733779No Hit
CTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCC545050.1454056199212075No Hit
AGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACAC542680.1447733635791962No Hit
GAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAA539280.14386632916449643No Hit
CTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGA534630.1426258262149806No Hit
CTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGA534450.1425778067459671No Hit
CCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATC514360.1372183004544029No Hit
CAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTG506150.13502807911773082No Hit
GAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAGAGATTAGGA499460.13324335551939512No Hit
CATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCA498260.13292322572597165No Hit
GGCATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACC496760.13252306348419235No Hit
GAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCC494780.13199484932504368No Hit
AGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCAGTTATT489290.13053025552013142No Hit
GCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTG475050.12673138197150652No Hit
CCTGACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGC474600.12661133329897273No Hit
ATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCAC474060.12646727489193219No Hit
CTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCT459440.1225670269087232No Hit
GTAATAAGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGA459210.12250566869831704No Hit
AGAGTGAATTCTGTCCCAGATCCACTGCCGCTGAACCTTGATGGGACCCC447190.11929903526752554No Hit
ACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCATCAG445870.11894689249475975No Hit
AAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAG441860.11787712543506973No Hit
ATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAGGCCAACCA419880.11201341471886361No Hit
CAGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAG417520.11138382612513084No Hit
AGTTGCAAAATCATCAGGCTGCAGGCTGCTGATGGTGAGAGTGAATTCTG412350.11000460026513148No Hit
CTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCGC409540.10925496299886493No Hit
GCTGCAGGCTGCTGATGGTGAGAGTGAATTCTGTCCCAGATCCACTGCCG401720.10716878384505546No Hit
AGATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGG389660.10395147942114985No Hit
GTTTGATGTCCACCTTGGTCCCTTGGCCGAACGTCCGCGGAGAATAAGAA384200.10249488886107316No Hit
GACTGCTGCCGATACCAGGCCAACCAGTTATTTACACTCTGACTGGCCCG380590.1015318317325243No Hit
GAGGCATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATA378670.10101962406304679No Hit
ATCCACTGCCGCTGAACCTTGATGGGACCCCACTTTCCAAAGTGGAGGCA376160.10035001924513609No Hit
CATCAGAGATTAGGAGTTTAGGGGCTTTTCCTGACTGCTGCCGATACCAG375690.10022463507604525No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)