FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005858132

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858132
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences384479427
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA18909731549.18268747836019TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA212989545.539686262589026TruSeq Adapter, Index 2 (100% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA40050721.0416869457101017TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA39862131.036781871816512TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA38783641.0087312161958668TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA38089540.9906782346510311TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA36931380.9605554265456185TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA36912160.9600555298372312TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA36739400.9555621814844204TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA35577460.9253410586257454TruSeq Adapter, Index 2 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA32675640.8498670593368316TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA30875830.8030554519110851TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA27916230.7260786413937306TruSeq Adapter, Index 2 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC16444030.4276959661615392TruSeq Adapter, Index 2 (100% over 63bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAC10848850.2821698441617788TruSeq Adapter, Index 2 (100% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACA8105610.21082038285497132TruSeq Adapter, Index 2 (100% over 62bp)
NATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA5435770.14137999638664672TruSeq Adapter, Index 2 (98% over 63bp)
ATTGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4679030.12169779893060441TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4564560.11872052649516668TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTTCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4465550.11614535619873362TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCATCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4305050.11197088056417645TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTTTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4197180.10916526880903825TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACATGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4162600.10826587088104457TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTTACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4162360.10825962867448821TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCTAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4152530.10800395829761783TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAATTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA4059590.1055866638086724TruSeq Adapter, Index 2 (98% over 62bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACAA3849890.10013253583006407TruSeq Adapter, Index 2 (100% over 62bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA251994400.067.016791
ACACGTT5874200.059.85067413
CTCCAGT268129700.059.72525424
AACTCCA268222950.059.70834722
GAACTCC268891650.059.69034621
TCACCGA266888250.059.6766730
CCAGTCA267765200.059.66540526
TCCAGTC268159950.059.66237625
CACCGAT266792150.059.66189231
AGTCACC267339800.059.6559528
GTCACCG266923550.059.65303829
ACCGATG271623900.059.64722432
TGAACTC274409800.059.6444720
CAGTCAC268062600.059.63735227
ACTCCAG268467500.059.62446623
TTACCGA5193000.059.6058330
CATCGAT5230050.059.59543631
CTGAACT274504000.059.5916919
ACACGTC270899200.059.57097213
CCGATGT272208150.059.56550233
Produced by FastQC (version 0.11.5)