FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005858138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30474321
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1031127033.83593025747809TruSeq Adapter, Index 10 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15044394.93674329938311No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG14132604.637543852084514TruSeq Adapter, Index 10 (100% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2963310.9723957426319688TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG2795560.9173493972187271TruSeq Adapter, Index 10 (100% over 63bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2110010.6923895039367735TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1964120.6445164110465332TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1803820.591914746845385TruSeq Adapter, Index 10 (98% over 63bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1773520.5819719494324418TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGTTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1390630.4563284609360123TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1356760.4452141854120392TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1298780.42618833082449975TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1294300.42471824064595237TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1284080.42136459742614113TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG1225620.4021812331766145TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATATCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1088020.35702846340694516TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCATACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG920990.3022183824866844TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATT911180.29899927876982063TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG676420.22196392825290512TruSeq Adapter, Index 10 (98% over 63bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG652860.21423282901036578TruSeq Adapter, Index 10 (98% over 63bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG579790.19025526442410318TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAGG533060.17492104253938914TruSeq Adapter, Index 10 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTAACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG502160.16478135804896193TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA469370.15402147926446005TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTTACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG456000.14963417888785774TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAAGG412800.13545830930900807TruSeq Adapter, Index 10 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG400530.13143196857445977TruSeq Adapter, Index 10 (98% over 63bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG391610.12850491402253064No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG387510.1271595189930565No Hit
GCTCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATG355210.11656043132183323TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTATTCTGCTTGAAAAAAAAAAGG326150.10702453386902369TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAAG325350.10676201776571168TruSeq Adapter, Index 10 (98% over 62bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG323990.1063157403900812TruSeq Adapter, Index 10 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGT318130.10439280993332059TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG311920.10235502868136094TruSeq Adapter, Index 10 (100% over 63bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)