FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005858140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21869564
Sequences flagged as poor quality0
Sequence length75
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3885471.7766563613248072TruSeq Adapter, Index 8 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3331171.5231990907546213No Hit
AGTATTATATTGTGGGGGTAAGAGTGTAATTTGGTTAGTAATGTGAAGATCGGAAGAGCACACGTCTGAACTCCA567050.25928729077543566Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATATTGTTAGTTAAATTGTGTTTTTGTTTTCACAGTATGATGCTAGATCGGAAGAGCACACGTCTGAACTCCA529310.24203043096789675Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGTATTATATTGTGGGGGTAAGAGTGTAATTTGGTTAGTAATGTGATGCTAGCCAAATTGCGCTCTTGCCCCCAC310950.14218390453508814No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310900.14216104171075383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG289030.1321608423469256TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAAGG221430.10125030384693541TruSeq Adapter, Index 8 (98% over 63bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG889250.067.767346
GCCGTCT757400.067.76729649
CGTCTTC743850.067.7634551
TCTCGTA817500.067.7437741
TGCCGTC838750.067.74261548
ATGCCGT849000.067.74151647
CCGTCTT762200.067.7388350
CTCGTAT826900.067.7246442
ATCTCGT808250.067.68674540
AATCTCG812750.067.655839
CGTATGC886050.067.45138544
GTATGCC890100.067.4157845
GAATCTC819450.067.4141738
CTGCTTG687100.067.24528557
CTTCTGC705350.067.07052654
GTCTTCT690700.066.9447452
CTTGAAT890700.066.8594135
CCGTCGT84950.066.84476550
GCCGTCG85100.066.8080249
TGAATCT834200.066.7930337
Produced by FastQC (version 0.11.5)