FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005858145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22178618
Sequences flagged as poor quality0
Sequence length75
%GC33

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCATCATACTGTGAAAACAAAAACACAATTTAACTAACAATATAAAGATCGGAAGAGCGTCGTGTAGGGAAAGA422600.1905438833023771Illumina Single End PCR Primer 1 (100% over 29bp)
TCACATTACTAACCAAATTACACTCTTACCCCCACAATATAATACTAGATCGGAAGAGCGTCGTGTAGGGAAAGA420160.18944372458193742Illumina Single End PCR Primer 1 (100% over 29bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG236500.10663423663277848No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)