FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005858149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22708574
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1538180.6773564909888221No Hit
TCACATTACTAACCAAATTACACTCTTACCCCCACAATATAATACTAGATCGGAAGAGCGTCGTGTAGGGAAAGA602820.2654592049681323Illumina Single End PCR Primer 1 (100% over 29bp)
AGCATCATACTGTGAAAACAAAAACACAATTTAACTAACAATATAAAGATCGGAAGAGCGTCGTGTAGGGAAAGA554000.24396071721632545Illumina Single End PCR Primer 1 (100% over 29bp)
AGCATCATACTGTGGGGGCAAGAGCGCAATTTGGCTAGCATCACATTACTAACCAAATTACACTCTTACCCCCAC361880.15935831109430296No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)