FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005858152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10963161
Sequences flagged as poor quality0
Sequence length75
%GC59

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG191629717.479420397091676No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4116213.754583190012443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG3006692.742539309602404TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG1651171.5061075906848398TruSeq Adapter, Index 8 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAAGG873150.7964400048489665TruSeq Adapter, Index 8 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG599380.5467218806692705No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCGTGAAAAAAAAAGGG571480.5212730160580511TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAAGG471640.4302043908686555TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAAGG453540.4136945539703376TruSeq Adapter, Index 8 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG310360.28309353479347793No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG292670.26695767762600586No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCGGCTTGAAAAAAAAAGGG285280.2602169210139302TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCGGCGTGAAAAAAAAAAGG281200.25649536661917127TruSeq Adapter, Index 8 (98% over 60bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCTGCTTGAAAAAAAAAGGG275690.2514694438948767TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCTGCGTGAAAAAAAAAAGG266180.24279493843062236TruSeq Adapter, Index 8 (98% over 60bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG251360.2292769393790714No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAAGG248280.22646753066930242TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCGGCGTGAAAAAAAAAGGG193380.17639073256335466TruSeq Adapter, Index 8 (98% over 60bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCTGCGTGAAAAAAAAAGGG182990.1669135388963092TruSeq Adapter, Index 8 (98% over 60bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGG175050.1596711021574891No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCGTGAAAAAAAAAAGG165060.15055876676443955TruSeq Adapter, Index 8 (98% over 60bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCGGCTTGAAAAAAAAAAGG153340.13986841933635746TruSeq Adapter, Index 8 (98% over 57bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCGGCGTGAAAAAAAAAAGG151800.13846371498147297TruSeq Adapter, Index 8 (98% over 57bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCTTGAAAAAAAAAGGG147410.1344593954243671TruSeq Adapter, Index 8 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGG142690.1301540677912146No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCTTCTGCTTTAAAAAAAAAAGG139230.1269980437211494TruSeq Adapter, Index 8 (98% over 62bp)
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131330.11979209280972888No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGG130770.11928129122613451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG129360.11799516581029869No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCGCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG112400.1025251749928693TruSeq Adapter, Index 8 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGGCTTCTGCGTGAAAAAAAAAGGG111980.10214207380517352TruSeq Adapter, Index 8 (98% over 60bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCCGTCGTCGGCGTGAAAAAAAAAGGG110530.10081946256193812TruSeq Adapter, Index 8 (98% over 57bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)