FastQCFastQC Report
Wed 27 Apr 2022
EGAF00005858154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18249114
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG7004303.838158937469512TruSeq Adapter, Index 4 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4129112.2626358737196774No Hit
AGTATTATATTGTGGGGGTAAGAGTGTAATTTGGTTAGTAATGTGAAGATCGGAAGAGCACACGTCTGAACTCCA1729870.9479199921705789Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TTATATTGTTAGTTAAATTGTGTTTTTGTTTTCACAGTATGATGCTAGATCGGAAGAGCACACGTCTGAACTCCA1677920.9194528567249896Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGAGGATAGGTTTTTGGAAAGGTTGTTTATTTTTTGAGTATTAGATCGGAAGAGCACACGTCTGAACTCCAGTCA803560.44032822634567353Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGTATTTAAAGAATAGGTGATTTTTTTGAGAATTTGTTTTTCAGATCGGAAGAGCACACGTCTGAACTCCAGTCA792720.43438821194278254Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGG626000.34303035204887206TruSeq Adapter, Index 4 (100% over 63bp)
AGTATTATATTGTGGGGGTAAGAGTGTAATTTGGTTAGTAATGTGATGCTAGCCAAATTGCGCTCTTGCCCCCAC476390.2610482897964252No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG444030.24331592207709368No Hit
GGTATTTAAAGAATAGGTGATTTTTTTGAGAATTTGTTTTTGGAAAGGTCGCCTATTCTTTGAGTACCAGATCGG294570.16141605559590455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG277190.15189230556617708No Hit
CGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG270820.14840172514676603No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG246660.13516272625618975No Hit
GGAGGATAGGTTTTTGGAAAGGTTGTTTATTTTTTGAGTATTTGTCCTCCAGATCGGAAGAGCACACGTCTGAAC205360.11253149056989836Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)