FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005858159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005858159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34179093
Sequences flagged as poor quality0
Sequence length75
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1690270.49453331017297625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGATT24350.012.3199621
GTGTAGA117400.012.1411769
GATCGCG14200.011.8956414
TTGAACG31200.011.83143725
TGAACGC23900.011.54794926
TAGGGAA549350.010.66132769
GAGCGCA18650.010.54371122
GTAGGGA597500.010.30470469
AGTGTAG151550.010.17939368
CGAGTAG41950.09.6170741
CGGAGTC18950.09.4620061
AGCGCAA21850.09.15735923
TGTAGGG687950.09.04501168
CGCTCTG31250.08.9376651
GTGAGTT93850.08.89526420
GCGCAAT21150.08.80813724
GAGTGTA163850.08.78351169
CGCCTCG33850.08.7604991
TGAGTTG101350.08.74774321
CGTGATC29700.08.7075081
Produced by FastQC (version 0.11.5)