FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19980078
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1215600.6084060332497201TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG137100.041.6184443
CGTATGC140550.040.69024744
GATCGGA142000.040.6010481
TCTCGTA138700.039.6949741
ATCGGAA147500.038.9704632
ATCTCGT142550.038.6682140
AATCTCG143150.038.3212739
CGTCTGA151250.038.21580516
ACGTCTG154450.037.43826715
CACGTCT159150.036.42938214
ACACGTC164700.035.1379313
GACCAAT158100.034.6016835
GCACACG170800.034.02500511
CTCGTAT169200.033.89157542
ACCAATC164200.033.6079236
CGGAAGA172550.033.593414
CACACGT174700.033.1644512
TCGGAAG175800.032.947343
ACTGACC173550.032.79180532
AGAGCAC191450.030.4124818
Produced by FastQC (version 0.11.5)