FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011767

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011767
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19827150
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC1154090.5820755882716376TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG130450.041.1856343
GATCGGA135250.040.440211
CGTATGC133950.040.256744
TCTCGTA130450.039.4045441
ATCGGAA139600.039.055972
ATCTCGT132200.038.9819140
AATCTCG135350.037.97714239
CGTCTGA144200.037.7228416
ACGTCTG146300.037.36084415
CACGTCT152700.035.86707714
ACACGTC156500.034.88480413
GACCAAT151000.034.2594835
CTCGTAT157850.034.13994642
CGGAAGA161700.033.8995974
GCACACG162800.033.6023911
ACCAATC153800.033.5343836
TCGGAAG168300.032.552933
CACACGT168750.032.40456412
ACTGACC167150.032.3851932
TGACCAA180300.030.18035534
Produced by FastQC (version 0.11.5)