FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27692649
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC3602171.300767579150698TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA412150.042.5654261
CGTATGC404850.042.3281944
TCGTATG405050.042.2934543
TATCTCG394150.041.8033739
ATCGGAA422300.041.575732
TCTCGTA398100.041.38165341
ATCTCGT401700.041.0874540
CGTCTGA424750.040.91425316
ACGTCTG428900.040.68249515
CACGTCT437400.039.92889814
ACACGTC441950.039.4929313
GCTTATC415650.039.44857436
CTCGTAT434600.039.44307342
CACTAGC439100.039.19373731
TAGCTTA436750.039.19079634
CGGAAGA449850.039.027624
TCGGAAG453850.038.8357963
ACTAGCT442750.038.7888932
GCACACG452800.038.7501411
CACACGT456950.038.37951712
Produced by FastQC (version 0.11.5)