FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25192342
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1889200.749910429129614TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG220700.041.80209743
GATCGGA224950.041.4239161
CGTATGC225700.040.88489544
TCTCGTA217550.040.35434341
ATCGGAA231850.040.0717472
ATCTCGT221500.039.6534740
CGTCTGA236300.039.5037916
AATCTCG224000.039.1318839
ACGTCTG239750.039.0454215
CACGTCT248700.037.69335614
CTCGTAT253200.036.50564242
GCACACG261800.035.88535311
ACACGTC262100.035.77685513
CGGAAGA268700.035.1328474
CACACGT275700.034.05204812
TTGAATC258000.034.04134836
GAATCTC264100.033.33949338
TCGGAAG282350.033.3096773
TCTGAAC289550.032.3527518
ACACTTG285700.032.33809732
Produced by FastQC (version 0.11.5)