FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24924786
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC1791890.7189189106779091TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG204100.041.62098743
GATCGGA210650.041.060251
CGTATGC208300.040.85546544
ATCGGAA215750.040.0187382
TCTCGTA204600.039.80414241
ATCTCGT206500.039.45826740
CGTCTGA221950.038.92862316
AATCTCG209200.038.8964239
ACGTCTG226100.038.1832315
CACGTCT233400.037.04552514
CTCGTAT235100.036.1942742
ACACGTC245400.035.20754213
GCACACG245500.035.19320311
CGGAAGA255200.034.3753624
TTGAATC244100.033.53260836
CACACGT258750.033.51854712
TCGGAAG266000.032.8978773
GAATCTC251400.032.54179438
AGAGCAC270350.032.0717058
ACACTTG268450.031.91373432
Produced by FastQC (version 0.11.5)