FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59922957
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2261870.3774630147173812No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1658060.2766986282068824No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT786080.13118177729446828No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCCCC796050.020.7010361
ACGTTCG63250.017.929256
CGTGCGA70250.016.34212338
GCGATCG71000.016.0710241
CGCGTAA35550.016.0466570
CGTTCGA73500.015.6183787
CGATCGG81050.014.46691742
CCCCCCT461700.013.9578333
CCCGTAT63200.013.9397011
GTCCGTT54550.013.7050831
GTCCGAT69650.013.5054931
CGTATCG49100.013.1177823
TCGACCG61600.012.89661427
TCGCGTA44250.012.73355569
GCGTGCG97350.012.11629437
GTATCGA38650.011.9536934
GTTCGAA101200.011.7929738
GTTCGAC69450.011.74124725
GTCGGAT47500.011.6011981
CGACCGT69350.011.50537728
Produced by FastQC (version 0.11.5)