FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011777

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011777
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59922957
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1349120.22514242746732274No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCCTTT768400.018.9979577
CCCTTTT842650.017.6211458
CCCCCTT771500.017.3479826
CCCCCCT787200.015.40696055
CCTTTTT1008850.014.85039
GTACGCA97000.013.50373150
TACGCAC102950.012.62232851
ACGCACG106300.012.58263652
CCGGTAC106200.012.4629560
AGTACGC106750.012.39973149
TAGCATA129500.011.7939339
CGGTACA111750.011.7786961
TAGCGTA48000.011.70979837
GCCGGTA116100.011.58041659
CTTTTTT1418600.010.97222310
CGCACGG128100.010.79597853
CGCGAGT57150.010.70021113
CGCACGA59250.010.28213628
TTCGTCG60150.010.2150838
GCGTATA56450.010.01900339
Produced by FastQC (version 0.11.5)