FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006011780

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006011780
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63590261
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1212940.19074304475649187No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1000620.1573542841725402No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG73850.017.6775726
CCCCCCC794850.016.1262841
CGTTCGA82000.015.9615567
CGTGCGA81950.015.92905338
GCGATCG82600.015.71938641
CGCGTAA51650.013.55298170
CGATCGG101300.013.16322742
TCGACCG83800.012.32061427
GTTCGAA110550.012.0921348
CCCGTAT61550.011.9882531
GCGTGCG114700.011.89951537
CGCGTTA64550.011.81982834
CGACCGT88400.011.71967728
TCGCGTA60400.011.64754369
GTCGGAT58950.011.5633211
CCCCCCT422100.011.5192423
GTCGCTA53700.011.47077527
GTCCGTT58050.011.3794241
GTTCGAC92650.011.143537525
CGTATCG59150.010.9484843
Produced by FastQC (version 0.11.5)