FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040558

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040558
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29681464
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCACA4341871.4628220494784219No Hit
GAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACAT1794040.6044311021855256No Hit
TATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGC1708850.5757296877269935No Hit
ATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCA1592960.536685117688265No Hit
ATTTATACTGTTGATATGGCATAGCTGTGCAGTACGAGAGGAACCACAGC1487130.5010298683380308No Hit
TGTTGATATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACC1483860.4999281706589675No Hit
CAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACATTGGTATGAA1380920.4652465929578136No Hit
TGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCAC1302580.43885301614502575No Hit
GGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACATTG1169540.39403042922680637No Hit
GCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCACAA1150680.3876762952123925No Hit
CACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACATTGGTATG1149470.38726863338007855No Hit
AGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACA1059260.35687592768335147No Hit
CATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCACAAT988200.33293506007655144No Hit
CGAGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGA959610.32330278587336525No Hit
GTTGATATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCT825110.2779883094715274No Hit
TTTATACTGTTGATATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCT695410.23429100397473657No Hit
AGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACATT666430.22452733463551527No Hit
ACGAGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGG653640.22021824799477546No Hit
ATATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGG607000.2045047373674021No Hit
GAGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGAC555620.1871942704712948No Hit
GTACGAGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGC480010.16172045961075235No Hit
CGAGGAGCTATCATAGAGAAGCCGATGGGAGGTATCGGAAGAGTTTTCTT455590.15349310263132573No Hit
CATTTATACTGTTGATATGGCATAGCTGTGCAGTACGAGAGGAACCACAG448030.15094605845587672No Hit
GCTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCACAATACTT445860.1502149624425534No Hit
CGGAAGAGTTTTCTTTTCTGTTTTACAATCGTACGTACCATGGAAGTCTT413550.13932938078795573No Hit
CTACAAACCTATGGCACAATACTTGTCCGAGCGGACATTGGTATGAAGCT407710.1373618228534819No Hit
CACGCGTGTTACAATGAAGCTGACAACGTGCTCTCCTTATCCGAGAGGAT398240.13417127942206625No Hit
ACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACATTGGTA394990.13307631995510733No Hit
GTTAAAGATTAATGACATCTCGGTAACGAGGAGCTATCATAGAGAAGCCG393790.13267202722884558No Hit
TACGAGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCG391410.13187017998842646No Hit
GATTAATGACATCTCGGTAACGAGGAGCTATCATAGAGAAGCCGATGGGA385430.12985545456922204No Hit
TTATACTGTTGATATGGCATAGCTGTGCAGTACGAGAGGAACCACAGCTA382250.12878407884462842No Hit
CGCGTGTTACAATGAAGCTGACAACGTGCTCTCCTTATCCGAGAGGATTG372950.12565081021609986No Hit
GCGAAACAGGATTAGCCCATCACGTAGGATCGTAGGCTTGATTGTCTACT355190.11966727786742594No Hit
CAAACCTATGGCACAATACTTGTCCGAGCGGACATTGGTATGAAGCTACG351940.11857231840046703No Hit
AAGAGGTGCAGCATAGGTGGGAGTATAGCAATATACACCAATGAGATACC346050.11658791493573228No Hit
GAGGTATCGGAAGAGTTTTCTTTTCTGTTTTACAATCGTACGTACCATGG330750.11143318267589496No Hit
CTGTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCACAATACTTG321430.10829317583526203No Hit
GTGCAGTACGAGAGGAACCACAGCTACAAACCTATGGCACAATACTTGTC319010.10747785217063417No Hit
GAACCACAGCTACAAACCTATGGCACAATACTTGTCCGAGCGGACATTGG315890.10642669108235363No Hit
TACAAACCTATGGCACAATACTTGTCCGAGCGGACATTGGTATGAAGCTA306280.10318898016620744No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATA290050.051.5492251
TTTATAC348600.043.1639332
TTATACT387600.038.8813
TATACTG397950.037.9483834
ATACTGT436950.034.6208085
TACTGTT436450.034.617176
CATTTAT153350.028.696811
TCCTGGC93350.024.4158867
ACTGTTG631800.024.0401297
CCTGGCT96850.023.9701188
GGGATTC87300.023.7724631
GGCATAG1829100.023.669751
CTGTTGA678900.022.6767568
GCATAGC1981150.021.8610292
CTGGCTC109350.021.6597739
GTTTGAT132850.020.9827161
CATAGCT2064050.020.9781633
ATTGTCC156050.020.5081378
TAGCTGT2153200.020.1449095
CTGTGCA2217300.019.6110558
Produced by FastQC (version 0.11.5)