FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45961554
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG177350.048.49732294
TAAGTCG45100.026.78270394
CAAGTCG151350.026.73736294
GAAGTCG175200.018.42979494
CGTCGGA132300.018.18896394
AATAGCG60450.014.38378890-91
GGCGGAT219800.014.3694261
CGTATCG63800.013.1128523
GTTCGAT70100.012.8059911
CGCTGTT179000.012.7871511
CGAATGT146150.012.6705438
GTCGGAA125400.012.4808621
GTCCGAT30800.012.360391
ATACGGA104950.012.15864756-57
TAGCGGG201700.012.11700594
CAACCCG67150.012.1087111
GCGGGTT67250.012.0907061
AGTTCGC91400.012.03282262-63
GGTTCGA76900.011.9791944
GACGCTA67150.011.9687264
Produced by FastQC (version 0.11.5)