FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49449904
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG168000.041.3488194
CAAGTCG133300.021.3668494
TAAGTCG53400.020.24344494
GAAGTCG207700.014.61819794
CGAATCG24250.014.5360823
GTTTTTT867500.014.2869161
GTCGGTT93750.014.2378651
GTTTAAT302250.013.4196841
GTCGATT36300.013.2066121
GTTTATT331400.012.94841
TTAGCGA45050.012.7280797
GTTTTAT460050.012.5353771
CGGGAAT124900.012.5308251
GTTTTCT689250.012.4787811
CGGAAAT121850.012.4201881
CGACTAT47400.011.7995781
CGAAAAT92400.011.6991331
AAATCCA582950.011.658294
GTTCGAT49700.011.631791
CGCAAAT61900.011.6171241
Produced by FastQC (version 0.11.5)