FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44634632
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG106100.042.1272494
TAAGTCG57400.024.89198794
CCCGGGT297750.024.798321
CGGGTTC377250.021.6903923
GAAGTCG161150.021.64070994
CCGGGTT385400.021.451222
GTCGGTT103600.019.1447891
GGTTCAA670900.017.3106295
GGGTTCA613800.016.455364
TTCAAGC571300.015.8695967
AAAGTCG81400.015.47420194
CAAGCGA319850.014.9588879
AACGTAT165400.014.492145
TCAAGCG317950.014.3091698
GAACGTA172200.014.0017424
TCGGTTT150350.013.7545722
CGTATTC175650.013.245097
CCGAGTA337000.013.1237394
CGAGTAG325500.013.0675895
ACGTATT190600.012.4527816
Produced by FastQC (version 0.11.5)