FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47305264
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGTCG97900.035.1899994
TAAGTCG68550.018.92341694
GTCGGTT167000.018.0119761
CCCGGGT309300.017.4141641
CGGGTTC403250.016.3990083
CCGGGTT413900.016.3517762
GAAGTCG183650.015.81595594
GGTTCAA576450.014.34995
TTCAAGC492000.013.7752037
TCGCTAT101150.013.5679682
GGGTTCA535850.013.3057774
TCGGTTT253850.013.2196192
AAAGTCG91250.012.77369894
CGCTATG107400.011.9031653
CGTCAAT182550.011.6631073
AACGTAT187550.011.5025335
GCCGGGT441000.011.4782321
CCGTCAA184850.011.441712
CGAGTAG302500.011.1246285
GCGTACG249100.011.11320814-15
Produced by FastQC (version 0.11.5)