FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040572

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040572
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21500544
Sequences flagged as poor quality0
Sequence length126
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1245990.5795155694665214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG323400.012.172279120
GTCGTGT245500.012.148228120
GCGTCGT286750.011.844618120
CGTCGTG262950.011.638716120
TCGTGTA220100.010.9055605120
AGAGCGT420650.010.870315120
CGTGTAG211550.010.268419120
AAGAGCG477650.010.213835120
GAGCGTC383750.09.851451120
AGATCGG799250.09.467612120
CGGAAGA647200.09.448083120
GTGTAGG254150.09.397255120
ATCGGAA685300.09.351872120
GATCGGA727900.09.2579775120
TCGGAAG669700.08.673675120
TAGATCG106950.08.416248120
CAGATCG309350.07.9337997120
GTAGGGA229750.07.57444120
GAGATCG368700.07.5518417120
AAGATCG258350.07.223705120
Produced by FastQC (version 0.11.5)