FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77263437
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAT736850.07.08511831
CAAAAAT829950.06.5451271
CTAAAAT516950.06.3811761
TGAAATC531250.06.2094062
CTTAAAT403950.06.1653881
CTAAAAC348300.06.09813641
AAATCCA655400.06.00826644
CTGATTT645550.05.9398091
CTGAGAT731850.05.89430761
CTGGAAT730300.05.8424731
CTGAATT566000.05.74515631
CTCAAAT452300.05.64138031
CAAAAAC626350.05.6117291
GAAATCC475450.05.6038933
CTGAAAC585900.05.59814451
AATCCAG585050.05.51791955
AAATCTA403900.05.5029924
CTTAAAC268650.05.49230961
CTAAATT342200.05.4515841
AAAATCC538500.05.4451583
Produced by FastQC (version 0.11.5)