FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040578

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040578
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77263437
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAAAAT823050.06.8411331
CTAAAAT501650.06.8019471
CTGAAAT732950.06.6689341
CTGAGAT721050.06.0554691
CTTAAAT388150.06.04224541
AAATCCA678300.06.0421354
CTAAAAC330950.06.0356491
TGAAATC529550.05.9554092
CTGAATT546850.05.9131331
CTGAAAC587200.05.87499431
AAATCTA397550.05.8047764
CTGGAAT729100.05.7823281
CTCAAAT447300.05.7265761
AATCCAG591150.05.64489845
CTGATTT617250.05.58897831
CGAAAAT89050.05.3834871
CTTAAAC263800.05.3805861
GAAATCC488050.05.35434683
CAAAAAC654050.05.35356141
AAATTCA730450.05.3469524
Produced by FastQC (version 0.11.5)