FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25516069
Sequences flagged as poor quality0
Sequence length126
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG383050.011.42997120
TCGTGTA410150.011.245047120
GTCGTGT449750.010.49497120
GCCGTAT34750.010.356146-47
CGTCGTG487800.010.229613120
GCGTCGT524250.010.170468120
CGCCGTA27150.010.05174346-47
AGCGTCG581250.09.95731120
GAGCGTC663250.09.558185120
CGTATCA37600.09.49133348-49
AGAGCGT722850.09.46706120
AAGAGCG802200.09.106306120
GTGTAGG424000.09.06713120
GTAGGGA370150.09.041367120
TCGCCGT40300.09.00421444-45
TGTAGGG431750.08.807133120
GATCGGA1170550.08.064778120
TAGGGAA397700.07.977701120
CGGAAGA1029950.07.960311120
ATCGGAA1107500.07.8144827120
Produced by FastQC (version 0.11.5)