FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040593

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040593
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46430062
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG148750.047.3633694
CAAGTCG132250.024.69943294
TAAGTCG41700.022.65467694
CGTCGGA113500.022.11277494
GAAGTCG171100.016.34424494
GGCGGAT226250.016.2656361
GGGGAAT319100.013.0498281
GTTCGAT75400.012.6538471
TATAGGT135650.012.1960937
GCGGATG264450.012.1387792
CGGATGA231200.012.1159173
ATACGGA86600.011.99422656-57
ATATAGG172300.011.5113186
AATAGCG44600.011.48654790-91
GTCGGAA110300.011.4623761
GCGAGTA91300.011.35104150-51
GTCCGAT40100.011.1346641
GGCGGTT96250.011.0358441
AGCCGTA131200.011.01562582-83
TAGCGGG181700.010.73472894
Produced by FastQC (version 0.11.5)