FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040594

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040594
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46430062
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG162200.043.1171494
CAAGTCG140600.024.20199294
TAAGTCG43200.022.41203794
CGTCGGA119700.016.3341794
GAAGTCG177850.015.06325694
GGCGGAT207300.014.2836471
GTCCGAT39100.013.8235291
AATAGCG42450.013.2862290-91
GGGGAAT315550.012.7200131
GGCGATT111000.011.9828831
CGACCTA23950.011.9707734
CGTATCG52000.011.8403853
GTTCGAT71250.011.8077191
CGATTGG38750.011.6438714
GTCCGTT32900.011.4285721
GTCGGAA107850.011.2869731
TATAGGT123650.010.9850387
GGGGGAT291950.010.8021921
GGCGGTT98300.010.7578841
CGCGTAT10100.010.70297051
Produced by FastQC (version 0.11.5)