FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41426809
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG60500.028.9768694
GTCGATT34750.026.1035961
GCGACAT38950.018.462131
CGATTTG50200.015.3545823
TCGATTT67650.014.8677022
CGACATC55700.014.5978462
GTTTTTT1156450.014.0863851
GCCGAAT46600.013.9184541
TTGGCGA53050.012.4919887
CGAAAAT63800.012.3761751
CGATCCC109350.012.2926383
GTTTTAT541600.012.1144761
CGCGATT12450.012.0803221
CGCGATC130000.011.9669231
CGAATCC56700.011.60493853
TTGAACC493100.011.5426892
CTCGGAT62800.011.3757961
GTTTTTC538050.011.3470871
GCGGAAT31550.011.3217121
GAAGTCG72850.011.22580694
Produced by FastQC (version 0.11.5)