FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39287339
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG614050.011.359432120
TCGTGTA420750.011.16139120
CGTCGTG517200.010.703451120
GTCGTGT475950.010.686001120
GCGTCGT543350.010.431164120
CGTGTAG409600.09.8545265120
AGAGCGT784750.09.591643120
AAGAGCG894200.09.450547120
GAGCGTC730450.09.385046120
GATCGGA1328750.09.158395120
ATCGGAA1245600.09.134176120
TCGGAAG1231900.08.817057120
GTGTAGG492850.08.725393120
CGGAAGA1195000.08.517156120
AGATCGG1451750.08.473336120
CAGATCG613100.08.070553120
TAGATCG175050.07.503478120
GTAGGGA449750.07.467883120
TGTAGGG528900.07.30286120
AAGATCG461400.07.2403283120
Produced by FastQC (version 0.11.5)