FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35142541
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG381170.10846398386502559No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGGTT199300.020.18661
TCGAGTT246750.018.03961824-25
GGGGGGT226500.017.5128381
ATCCGGA237500.017.36013264-65
AATCGCA263500.017.32815246-47
AGTCGAG257750.017.22024522-23
ACATCCG259400.017.1150562-63
GCAGTCG266850.016.75784120-21
GGGGGAT251250.016.6689551
GAAATCG269750.016.50174344-45
GGGGGGA298900.015.934481
GGGGTAT111250.015.6256821
GTCGAGT237350.015.57892424-25
GCGGGTT46900.015.5693861
CCGGAGT262150.015.50623566-67
GGGGGCT459650.015.3633891
TCGCAGG291250.015.30197148-49
CAGTCGA248650.015.242352522-23
CCGATCG200750.015.15954574-75
GTCGGTT20400.015.0105231
Produced by FastQC (version 0.11.5)