FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040602

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040602
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77274260
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAAAT739400.07.13834331
CAAAAAT853150.06.8311441
CTAAAAT523650.06.46232131
CTGAGAT724200.06.1524341
CTAAAAC353000.06.12463571
AAATCCA700100.06.00168854
CTGGAAT729300.05.99985361
CTTAAAC273500.05.9286931
CTTAAAT409150.05.9274011
CTGAAAC605700.05.85074141
AATTAGC326700.05.81203175
TGAAATC533850.05.7929892
CTGATTT649150.05.7559791
AAAATCC576900.05.74360563
CTCAAAT465900.05.74006371
GAAAATC554550.05.68692972
CTGAATT568650.05.6864411
CAAAAAC661800.05.6814651
AAATCTA409050.05.6186054
AAAAATC764600.05.5630162
Produced by FastQC (version 0.11.5)