FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040606

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040606
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22330014
Sequences flagged as poor quality0
Sequence length126
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1191700.5336763335661142No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTGTA97100.010.258894120
GATCGGA299900.010.24484120
CGTCGTG113500.09.93971120
AGCGTCG129800.09.847287120
ATCGGAA285500.09.458408120
GCGTCGT114900.09.348559120
AGATCGG337350.09.303206120
CGGAAGA309600.08.838439120
GTCGTGT111400.08.834264120
AGAGCGT185300.08.77618120
TCGGAAG306600.08.729199120
GAGCGTC175300.08.72911120
CGTGTAG104000.08.48195120
AAGAGCG221150.08.1946745120
CAGATCG151200.07.5010433120
TAGATCG61400.07.232366118-119
AAGATCG143000.06.8820763120
GAGATCG199650.06.0714684120
Produced by FastQC (version 0.11.5)