FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040607

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040607
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42077849
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG119800.057.161194
CGACGGT41950.036.5244331
TAAGTCG79350.034.11720394
GTATCGA49550.031.6811316
CGGTATC50000.031.1144
TATCGAT53550.029.4024287
GGTATCG53600.029.111945
ACGGTAT54400.028.6838253
GACGGTA54450.028.484852
ATCGATA55750.028.2421538
TCGATAA66050.024.3361079
GATATCG46700.023.449685
ATATCGA49050.022.613666
CGCTCTA67300.020.00817352-53
CGATTGA53950.019.16589490-91
TTAACCC175650.018.8909782
CTATCGG42950.018.82188660-61
TATCGGA44100.018.81065860-61
GAAGTCG120600.018.3167594
TCTATCG46950.017.86900958-59
Produced by FastQC (version 0.11.5)