FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41917370
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATT33450.024.7294481
CGATCCC156150.023.8988133
CGCGATC172250.022.6473141
GCGATCC210250.017.0340062
GCGACAT40900.016.0880181
CGCGATT24900.015.2891561
CGATTTG51350.015.2852963
CCCACTA258400.014.7511617
CACTACT266650.013.924629
TCGATTT68300.013.9004382
GTTTGAT253050.013.7443191
CCACTAC285500.013.7131358
GTTTTTT1041700.013.5671491
AAGTCGG87600.013.52054894
ATCCCAC327950.013.0846175
CTCGAAT60100.012.9816971
TTGGCGA58650.011.9403247
TTGAACC461550.011.8938352
GATCCCA347400.011.82440954
GTTTTAT479450.011.7732811
Produced by FastQC (version 0.11.5)