FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71649133
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG169700.068.18738694
CAAGTCG208500.031.0628394
GAAGTCG364200.030.00411894
AAAGTCG159000.025.95345994
CTAAGTC257000.018.06848394
ATAAGTC209100.014.85748594
TTAAGTC231150.012.80986494
ACTAAGT156950.08.05543294
GTAAGTC254400.07.408411594
ACAAGTC300950.07.40255894
GGAAGTC585400.06.856508394
AGAAGTC617450.06.751801594
TCTAAGT234700.06.688538692-93
GCTAAGT179250.06.476429594
GATAAGT165650.06.043464794
CATAAGT183200.05.79803594
CCTAAGT218250.05.448339594
CGAAGTC196100.05.057113694
Produced by FastQC (version 0.11.5)