FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48874431
Sequences flagged as poor quality0
Sequence length126
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA1976500.08.885936120
ACACGTC2660700.08.854774120
ACGTCTG2169300.08.529164120
CACACGT2943000.08.363425120
CACGTCT2368950.08.267834120
GCACACG3215400.08.113668120
AGCACAC3620550.07.8626056120
GAGCACA3948500.07.856105120
TCTGAAC1953950.07.8144665120
GTCTGAA2090650.07.810375120
CTGAACT1921250.07.527533120
CGGAAGA5167000.07.3947787120
AGAGCAC4142200.07.3884373120
GATCGGA6013000.07.378718120
ATCGGAA5664750.07.342351120
AAGAGCA4614700.07.341706120
TCGGAAG5417800.07.3102875120
GAAGAGC4958950.07.2618556120
AGATCGG6416000.07.11044120
GAACTCC1951550.07.1083097120
Produced by FastQC (version 0.11.5)