FastQCFastQC Report
Wed 20 Apr 2022
EGAF00006040621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72073541
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGTCG188450.070.2568394
GAAGTCG331550.034.16377394
CAAGTCG177450.032.60467594
AAAGTCG144000.025.16458394
CTAAGTC268850.019.68458494
ATAAGTC234300.016.36875794
TTAAGTC273000.011.37985394
ACTAAGT186200.08.48120394
GTAAGTC262700.07.97944494
GCTAAGT194350.07.786982594
GGAAGTC561150.07.705604694
AGAAGTC605950.07.329812594
TCTAAGT261950.06.943691394
ACAAGTC307250.06.54711194
CCTAAGT225750.06.5373294
GATAAGT190200.06.536014692-93
TGAAGTC647650.05.333899594
CATAAGT197250.05.1229494
ATTTTTG1210850.05.08097651
TAGAGCC246100.05.06095083
Produced by FastQC (version 0.11.5)