FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040623

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040623
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46562371
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG137300.053.2301594
TAAGTCG41300.028.67796594
CAAGTCG112400.027.974294
CGTCGGA100150.026.98452294
GAAGTCG150250.021.74043394
GTTCGAT178900.019.9927331
GGTTCGA155000.019.0729054
TTCGATT197600.018.3385622
TCGATTC205650.017.2778993
GGGTTCG181500.016.8837453
AATAGCG59150.016.76584890-91
CGACCGT20150.016.5607931
GAGTATT195800.015.6986719
ATCGGTA37700.014.9602116
GTACGGA127900.014.51524670-71
CGATTCC252250.014.4586724
ATAGATG235450.014.392448
GCCCATT242100.014.3077241
CGGATGA197700.014.1451693
GGCGGAT258050.013.8787061
Produced by FastQC (version 0.11.5)