FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49569198
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG161650.043.6999794
TAAGTCG43100.023.77262194
GTAAAAC231750.023.606471
CAAGTCG131600.020.17857294
CGAAAAT124700.016.1315161
TAAAACC341100.015.8595712
AAATACG117650.015.3404163
TTAGCGA82900.015.3075997
AAACCCA454450.015.2133334
GTTTTCT721750.015.0035331
AACCCAG494000.014.6137655
ACTCGAA222850.014.5734894
GTCGGTT125150.014.3835391
TCTCGTG278200.014.2250185
TACGGGC116700.014.2167966
CAATCGG102250.014.2034234
AAAACCC445250.014.1131943
GTTTTTT944700.014.0049751
GAAGTCG192900.013.64437494
TCAATCG105700.013.5619693
Produced by FastQC (version 0.11.5)