FastQCFastQC Report
Sat 23 Apr 2022
EGAF00006040627

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006040627
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49355658
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTCGG143250.042.75113794
TAAGTCG49000.021.7734794
CAAGTCG120000.020.36666794
GTTTTTT927950.014.1615391
GTCGGTT73250.013.4102391
GTTTAAT302950.013.3576491
GAAGTCG181700.013.19207694
GTTTTAT487400.013.1627011
GTTTATT388000.012.9007731
CGCAAAT59350.012.7497891
GTCGATT39350.012.4218561
CAATCGG66050.011.954584
CGAAAAT94800.011.7500011
AATCGGG92400.011.755
GTTTTCT710150.011.6085331
CGGAAAT109350.011.5189761
CTTTATT496100.010.9234031
GTTTTTC540800.010.8982991
CGCGAAT20550.010.7493921
GTCGGAT70350.010.689411
Produced by FastQC (version 0.11.5)